Selective Determination of Tetracycline in Broiler Chicken Meat by Molecularly Imprinted Polymer

Shendi Suryana; Aji Najihudin; Salma Elivania; Meillia Suherman

doi:10.24198/ijpst.v12i0.58889

Selective Determination of Tetracycline in Broiler Chicken Meat by Molecularly Imprinted Polymer

Shendi Suryana, Aji Najihudin, Salma Elivania, Meillia Suherman

Abstrak

Tetracycline (TC) residues in poultry meat products can create antibiotic resistance in humans who consume them. Hence, sensitive analytical procedures to evaluate the quantities of tetracycline residues are required to assess the safety of these products for consumption. The study aimed to develop a sorbent using molecular imprinting technology to analyze tetracycline in chicken broiler meat products. The study used several tests and computational techniques to increase the effectiveness of screening for the best MIP systems. According to the bond energybased computational analysis, methacrylate acid (MAA) was the best functional monomer at a TC-to-MAA molar ratio of 1:6. The mixture of methanol and chloroform yielded the greatest Ka. The Job plot showed that a TC-to-MAA-molar ratio of 1:6 was best for synthesizing imprinted polymer in the mixture of methanol and chloroform. We generated MIPs using two alternative production methods: bulk (MIP1) and precipitation (MIP2). Adsorption capacity results revealed that MIP1 matches well with the Langmuir model, whereas MIP2 fits better with the Freundlich. MIP1 application produced recovery rates of 82,74±4.1% and MIP2 results of 92.14±3.2% for TC in spike-chicken meat. The outcomes of the selectivity test also demonstrated that MIP2 is superior to MIP1 and can recover TC from spiked chicken meat while coexisting with another antibiotic drug. The study’s findings indicate that MIP2 helps determine TC in spike chicken meat.

Kata Kunci

Broiler chicken meat; molecularly imprinting polymer; tetracycline

Teks Lengkap:

PDF (English)

Referensi

Arabsorkhi B, Sereshti H. Determination of tetracycline and cefotaxime residues in honey by micro-solid phase extraction based on electrospun nanofibers coupled with HPLC. Microchem J [Internet]. 2018;140(April):241–7. Available from: https://doi. org/10.1016/j.microc.2018.04.030

Han S, Jin Y, Su L. Analytical Methods crystal sensor for highly efficient tetracycline. 2020;

Spectrometry C, Furi M, Sinaga S, Lux E De. Analysis of Amoxicillin and Tetracycline Residues in Chicken Meat Using High Performance Liquid. 2018;01(2):14–20.

Rukmana P.M., Eksan S, Karmana S, R.Y L. Residual content of Tetracycline HCl in Poultry Product (Meat and Liver) as a Result of Giving Feed Additive For The Whole Life. Media Vet. 2011;5 (1):17–22.

Singh SP, Pundhir A, Ghosh S. Validation of an analytical methodology for determination of tetracyclines residues in honey by UPLC-MS/MS detection. Indian J Nat Prod Resour. 2015;6(4):293–8.

Cui J, Xie A, Liu Y, Xue C, Pan J. Fabrication of multifunctional imprinted composite membrane for selective tetracycline and oil-in-water emulsion separation. Compos Commun [Internet]. 2021;28(October):100985. Available from: https://doi.org/10.1016/j. coco.2021.100985

Nofita N, Rinawati R, Qudus HI. Validasi Metode Matrix Solid Phase Dispersion (MSPD) Spektrofotometri UV untuk Analisis Residu Tetrasiklin dalam Daging Ayam Pedaging. J Kesehat. 2016;7(1):136.

Putri MA, Herawati D, Nety K. Pengembangan metode analisis antibiotik tetrasiklin dalam hati ayam menggunakan kromatografi cair kinerja tinggi (KCKT). Pros Penelit Sivitas Akad Unisba (Kesehatan dan Farm. 2015;2:79–85.

Andini A. Analisis Residu Antibiotik Tetrasiklin Pada Daging Ayam Broiler Dan Daging Sapi. J SainHealth. 2019;3(2):33.

Aniza SN, Andini A, Lestari I. Analisis residu antibiotik tetrasiklin pada daging ayam broiler dan daging sapi. J SainHealth. 2019;3(2):22.

Baazize-Ammi D, Dechicha AS, Tassist A, Gharbi I, Hezil N, Kebbal S, et al. Screening and quantification of antibiotic residues in broiler chicken meat and milk in the central region of Algeria. Rev Sci Tech. 2019;38(3):863-77.

Dhongade S, Pardeshi S, Kumar A. Molecularly Imprinted Microspheres for the Remediation of 2, 4Dichlorophenol from Wastewater Using ‘ Template Analogue Imprinting Strategy .’ 2017;11(2):17–23.

Zhao XF, Duan FF, Cui PP, Yang YZ, Liu XG, Hou XL. A molecularly imprinted polymer decorated on graphene oxide for the selective recognition of quercetin. Xinxing Tan Cailiao/New Carbon Mater [Internet]. 2018;33(6):529–43. Available from: http://dx.doi. org/10.1016/S1872-5805(18)60355-5

Bakhtiar S, Bhawani SA, Shafqat SR. Synthesis and characterization of molecular imprinting polymer for the removal of 2 - phenylphenol from spiked blood serum and river water. Chem Biol Technol Agric [Internet]. 2019;1–10. Available from: https://doi.org/10.1186/ s40538-019-0152-5

Krishnan H, Islam AKMS, Hamzah Z, Nadaraja P, Ahmad MN. A novel molecular imprint polymer synthesis for solid phase extraction of andrographolide. Indones J Chem. 2019;19(1):219–30.

Hasanah AN, Soni D, Pratiwi R, Rahayu D, Megantara S. Synthesis of Diazepam-Imprinted Polymers with Two Functional Monomers in Chloroform Using a Bulk Polymerization Method. 2020;2020.

Zamruddin NM, Herman H, Asman S, Hasanah AN. Synthesis and characterization of magnetic molecularly imprinted polymers for the rapid and selective determination of clofazimine in blood plasma samples. Heliyon [Internet]. 2024;10(13):e33396. Available from: https://doi.org/10.1016/j.heliyon.2024.e33396

Kartasasmita RE, Hasanah AN, Ibrahim S. Synthesis of selective molecularly imprinted polymer for solid-phase extraction of glipizide by using a pseudo-template. J Chem Pharm Res. 2013;5(10):351–5.

Hasanah AN, Rahayu D, Pratiwi R, Rostinawati T, Megantara S, Saputri FA, et al. Extraction of atenolol from spiked blood serum using a molecularly imprinted polymer sorbent obtained by precipitation polymerization. Heliyon [Internet]. 2019;5(4):e01533. Available from: https://doi.org/10.1016/j.heliyon.2019.e01533

Hasanah ANUR, Yulianti AB, Rahayu D. SELECTIVE ATENOLOL DETERMINATION IN BLOOD USING MOLECULAR IMPRINTED POLYMER WITH ITACONIC ACID AS FUNCTIONAL MONOMER. 2019;11(1).

Hasanah AN, Susanti I, Marcellino M, Maranata GJ, Saputri FA, Pratiwi R. Microsphere molecularly imprinted solid-phase extraction for diazepam analysis using itaconic acid as a monomer in propanol. Open Chem. 2021;19(1):604–13.

Nicholls IA, Karlsson BCG, Olsson GD, Rosengren AM. Computational strategies for the design and study of molecularly imprinted materials. In: Industrial and Engineering Chemistry Research. 2013. p. 13900–9.

Hammam MA, Abdel-Halim M, Madbouly A, Wagdy HA, El Nashar RM. Computational design of molecularly imprinted polymer for solid phase extraction of moxifloxacin hydrochloride from Avalox® tablets and spiked human urine samples. Microchem J [Internet]. 2019;148:51–6. Available from: https://doi.org/10.1016/j. microc.2019.04.063

Ahmadi F, Karamian E. Computational aided-molecular imprinted polymer design for solid phase extraction of metaproterenol from plasma and determination by voltammetry using modified carbon nanotube electrode. Iran J Pharm Res. 2014;13(2):417–30.

Suryana S, Mutakin M, Rosandi Y, Hasanah AN. Rational design of salmeterol xinafoate imprinted polymer through computational method: Functional monomer and crosslinker selection. Polym Adv Technol. 2022;33(1):221–34.

Suryana S, Mutakin M, Rosandi Y, Hasanah AN. Molecular Dynamic Study of Mechanism Underlying Nature of Molecular Recognition and the Role of Crosslinker in the Synthesis of Salmeterol-Targeting Molecularly Imprinted Polymer for Analysis of Salmeterol Xinafoate in Biological Fluid. Molecules. 2022;27(11).

Pratiwi R, Megantara S, Rahayu D, Pitaloka I, Hasanah AN. Comparison of Bulk and Precipitation Polymerization Method of Synthesis Molecular Imprinted Solid Phase Extraction for Atenolol using Methacrylic Acid. J Young Pharm. 2018;11(1):12–6.

Hasanah AN, Dwi Utari TN, Pratiwi R. Synthesis of Atenolol-Imprinted Polymers with Methyl Methacrylate as Functional Monomer in Propanol Using Bulk and Precipitation Polymerization Method. J Anal Methods Chem. 2019;2019.

Ahmad AL, Lah NFC, Low SC. Configuration of molecular imprinted polymer for electrochemical atrazine detection. J Polym Res. 2018;25(11).

Yang J, Li Y, Wang J, Sun X, Cao R, Sun H, et al. Molecularly imprinted polymer microspheres prepared by Pickering emulsion polymerization for selective solidphase extraction of eight bisphenols from human urine samples. Anal Chim Acta. 2015 May;872:35–45.

Hasanah AN, Rahayu D, Pratiwi R, Rostinawati T. Extraction of atenolol from spiked blood serum using a molecularly imprinted polymer sorbent obtained by precipitation polymerization. Heliyon [Internet]. 2019;(December 2018):e01533. Available from: https:// doi.org/10.1016/j.heliyon.2019.e01533

Cai W, Gupta RB. Molecularly-imprinted polymers selective for tetracycline binding. Sep Purif Technol. 2004;35(3):215–21.

Hasanah AN, Suryana S, Mutakin, Rahayu D. Evaluation performance of molecularly imprinted polymer prepared by two different polymerization method for atenolol recognition in human plasma. Asian J Chem. 2017;29(11):2429–33.

Hasanah AN, Yulianti AB, Rahayu D. Selective atenolol determination in blood using molecular imprinted polymer with itaconic acid as functional monomer. Int J Appl Pharm. 2019;11(1):136–43.

Susanti I, Triadenda AL, Murdaya N, Rahayu D, Pratiwi R, Rosandi Y, et al. Synthesis of multi-template molecularly imprinted polymers ( MT-MIPs ) for isolating ethyl para-methoxycinnamate and ethyl cinnamate from Kaempferia galanga L ., extract with methacrylic acid as functional monomer. 2024;

Ahmadi F, Ahmadi J, Rahimi-Nasrabadi M. Computational approaches to design a molecular imprinted polymer for high selective extraction of 3,4-methylenedioxymethamphetamine from plasma. J Chromatogr A [Internet]. 2011;1218(43):7739–47. Available from: http://dx.doi.org/10.1016/j. chroma.2011.08.020

Zhang K, Zou W, Zhao H, Dramou P, Pham-Huy C, He J, et al. Adsorption behavior of a computer-aid designed magnetic molecularly imprinted polymer via response surface methodology. RSC Adv [Internet]. 2015;5(75):61161–9. Available from: http://dx.doi. org/10.1039/C5RA10367C

Li H, Chen J, Tan L, Wang J. Solid-phase extraction using a molecularly imprinted polymer for the selective purification and preconcentration of norfloxacin from seawater. Anal Lett [Internet]. 2019;52(18):2896–913. Available from: https://doi.org/10.1080/00032719.2019. 1628245

Wang L, Fu W, Shen Y, Tan H, Xu H, McPhee DJ. Molecularly imprinted polymers for selective extraction of Oblongifolin C from garcinia Yunnanensis Hu. Molecules. 2017;22(4):1–14.

Li J, Zhang L, Fu C. The Recognizing Mechanism and Selectivity of the Molecularly Imprinting Membrane [Internet]. Molecularly Imprinted Catalysts: Principles, Syntheses, and Applications. Elsevier Inc.; 2016. 159182 p. Available from: http://dx.doi.org/10.1016/B978-012-801301-4.00008-6

DOI: https://doi.org/10.24198/ijpst.v12i0.58889