Antioxidant Activity of Extract Combination from Moringa oleifera Leaves and Garcinia mangostana L. Pericarp

Yesiska Kristina Hartanti; Diksa T. Lukas; Junius Hardy

doi:10.24198/ijpst.v12i0.59284

Antioxidant Activity of Extract Combination from Moringa oleifera Leaves and Garcinia mangostana L. Pericarp

Yesiska Kristina Hartanti, Diksa T. Lukas, Junius Hardy

Abstrak

Radical-related diseases can be mitigated through natural antioxidant therapies that help regulate the formation and accumulation of free radicals. The pericarp of Garcinia mangostana L. and the leaves of Moringa oleifera are well-known for their antioxidant properties due to their high level of bioactive compounds, such as flavonoids, phenolics, and xanthones. This study aimed to explore the antioxidant capabilities of these two herbal extract combinations. Using the maceration method with 96% ethanol, the extraction yields were 11.4% w/w for M. oleifera and 24.11% w/w for G. mangostana. Qualitative phytochemical screening revealed differences in secondary metabolites, particularly steroids and triterpenes, between the two extracts. The antioxidant activity was categorized based on EC50 values, with crude G. mangostana showing very strong activity (<50 µg/mL) and M. oleifera classified as strong (50–100 µg/mL). Combinations of the extracts (2–10 ppm) produced very strong antioxidant activity but showed reduced efficacy compared to G. mangostana alone, suggesting antagonistic interactions based on combination index (IC) calculation. This study emphasizes the need for multiple antioxidant assays to evaluate diverse mechanisms and recommends further research to optimize synergistic effects in herbal extract combinations.

Kata Kunci

Antioxidant; DPPH assay; Garcinica mangostana; Moringa oleifera combination

Teks Lengkap:

PDF (English)

Referensi

Trifena BE, Analisis uji in vitro dan in vivo ekstrak kombinasi kulit manggis (Garcinia mangostana L.) dan pegagan (Centella asiatica L.) sebagai krim antioksidan (Thesis). Depok: Universitas Indonesia; 2012.

Badan Pengawas Obat dan Makanan (BPOM), Daftar Obat Tradisional [diakses pada: 3 Januari 2025]. Tersedia dari: https://cekbpom.pom.go.id/produk-obattradisional.

Naing S, Sandech N, Maiuthed A, Chongruchiroj S, Pratuangdejkul J, Lomarat P, Garcinia mangostana L. Pericarp Extract and Its Active Compound α-Mangostin as Potential Inhibitors of Immune Checkpoint Programmed Death Ligand-1. Molecules. 2023;28:6991.

Chitchumroonchokchai C, Riedl KM, Suksumrarn S, Clinton SK, Kinghorn AD, Failla ML, Xanthones in Mangosteen Juice Are Absorbed and Partially Conjugated by Healthy Adults. The Journal of Nutrition. 2012;142(4):675–680.

Yasoob TB, Yu D, Khalid AR, Zhang Z, Zhu X, Saad HM, et al. Oral administration of Moringa oleifera leaf powder relieves oxidative stress, modulates mucosal immune response and cecal microbiota after exposure to heat stress in New Zealand White rabbits. J Animal Sci Biotechnol. 2021;12(66).

Peñalver R, Martínez-Zamora L, Lorenzo JM, Ros G, Nieto G, Nutritional and Antioxidant Properties of Moringa oleifera Leaves in Functional Foods. Foods. 2022; 11:1107.

Avilés-Gaxiola S, León-Félix J, Jiménez-Nevárez YB, Angulo-Escalante MA, Ramos-Payán R, ColadoVelázquez J, Heredia JB. Antioxidant and antiinflammatory properties of novel peptides from Moringa oleifera Lam. leaves. South African Journal of Botany. 2021;141:466–73.

Widyarman AS, Lay SH, Wendhita IP, Tjakra EE, Murdono FI, Binartha CTO. Indonesian Mangosteen Fruit (Garcinia mangostana L.) Peel Extract Inhibits Streptococcus mutans and Porphyromonas gingivalis in Biofilms In vitro. Contemp Clin Dent. 2019;10(1):123-8.

Ngibad K. Phytochemical Screening Of Sunflower Leaf (Helianthus annuus) and Anting-Anting (Acalypha Indica Linn) Plant Ethanol Extract. Borneo Journal of Pharmacy. 2019;2(1).

Gulcin İ, Alwasel SH. DPPH Radical Scavenging Assay. Processes. 2023;11(8):2248.

Smeriglio A, Iraci N, Denaro M, Mandalari G, Giofrè SV, Trombetta D. Synergistic Combination of Citrus Flavanones as Strong Antioxidant and COX-Inhibitor Agent. Antioxidants. 2023;12(4):972.

Departemen Kesehatan Republik Indonesia. Farmakope Herbal Indonesia. 2008;109-114.

Fatmawati A, Sucianingsih D, Kurniawati R, Abdurrahman M. Microscopic Identification and Determination of Total Flavonoid Content of Moringa Leaves Extract and Ethyl Acetate Fraction (Moringa oleifera L.). IJPST. 2021;1(1):66-74.

Shojaee MS, Moeenfard M, Farhoosh R. Kinetics, and stoichiometry of gallic acid and methyl gallate in scavenging DPPH radical as affected by the reaction solvent. Sci Rep. 2022;12(1):8765.

Maryam St, Razak R, Baits M, Salim A, Analysis of Vitamin C and Antioxidant Activity of Capsicum frutescens L. and Capsicum annuum L. (curly and large chili variety). IJPST. 2023;1(1): 57-64.

Rudiana T, Indriatmoko DD, Firginia K. Antioxidant Activity of The Combination of Ambarella Leaves (Spondias dulcis Parkinson) And Soursop Leaves (Annona Muricata Linn) Extract. JFSP. 2023;9(2):83-7.

Nakitto A, Byaruhanga Y, Wagner A, Muyonga J. Influence of ripeness stage on the bioactive compounds’ contents and antioxidant activities of Solanum anguivi Lam. fruits accessions. Heliyon. 2023;9(11).

Munteanu IG, Apetrei C. Analytical Methods Used in Determining Antioxidant Activity: A Review. Int J Mol Sci. 2021;22(7):3380.

Luís Â, Duarte AP, Pereira L, Domingues F. Interactions between the major bioactive polyphenols of berries: effects on antioxidant properties. European Food Research and Technology. 2017;244(1):175–85.

Nwachukwu ID, Sarteshnizi RA, Udenigwe CC, Aluko RE. A Concise Review of Current In Vitro Chemical and Cell-Based Antioxidant Assay Methods. Molecules. 2021; 26(16):4865.

DOI: https://doi.org/10.24198/ijpst.v12i0.59284