Pengaruh penambahan kitosan pada bahan basis gigi tiruan resin akrilik polimerisasi panas terhadap kekasaran permukaan dan jumlah Candida albicans: studi eksperimental laboratoris

Veronica Angelia, Siti Wahyuni, Saidah Ritonga, Venna Restuesa Amesta

Abstract


ABSTRAK

Pendahuluan: Resin akrilik polimerisasi panas memiliki kekurangan pada sifat mekanis sehingga diperlukan bahan penguat yaitu kitosan. Kitosan dapat meningkatkan sifat-sifat RAPP yang berkaitan dengan kekasaran permukaan dan jumlah Candida albicans. Tujuan penelitian menganalisis pengaruh penambahan kitosan 5, 6, 7, 8, dan 9% pada bahan basis gigi tiruan RAPP terhadap kekasaran permukaan dan jumlah Candida albicans. Metode: Jenis  penelitian eksperimental laboratoris. Sampel berjumlah 42 berukuran 12 x 12 x 3 mm untuk kekasaran permukaan dan 30 sampel berukuran 10 x 10 x 1 mm untuk Candida albicans yang dibagi menjadi 6 kelompok (kontrol, 5, 6, 7, 8, dan 9%) kemudian dicampurkan dengan RAPP dan di curing untuk menghasilkan basis akrilik. Sampel dikontaminasi dengan Candida albicans kemudian dihitung  koloni yang melekat pada permukaan basis  dengan  colony counter. Hasil perhitungan kekasaran permukaan dianalisis dengan uji One-way ANOVA and LSD sedangkan jumlah Candida albicans diuji dengan Kruskal-Wallis dan Mann-Whitney. Hasil: Nilai rerata kekasaran permukaan  kelompok kontrol sebesar 0,105±0,006; kitosan 5, 6, 7, 8, dan 9% sebesar 0,123±0,006; 0,137±0,005; 0,147±0,004; 0,158±0,005; 0,176±0,007 berturut-turut. Nilai rerata jumlah Candida albicans kelompok kontrol sebesar 250,60 ± 6,88; kitosan 5, 6, 7, 8, dan 9% sebesar 112,60 ± 4,78; 95,00 ± 2,00; 84,40 ± 3,29; 5,20 ± 1,64; 0,60 ± 0,90  berturut-turut dengan nilai p = 0,0001 (p<0,05). Simpulan: Penambahan kitosan 5% dapat dijadikan sebagai bahan penguat pada bahan basis gigi tiruan RAPP dengan nilai kekasaran permukaan tidak melebihi 0,2 μm sehingga dapat diterima secara klinis dan penggunaan kitosan 9% efektif mengurangi jumlah Candida albicans.

Kata kunci: 

resin akrilik polimerisasi panas, bahan penguat, kitosan, kekasaran permukaan, candida albicans

 

The effect of chitosan addition on polymethyl methacrylate denture base on surface roughness and against Candida albicans growth: a laboratory experiment study


ABSTRAK

Introduction: Polymethyl methacrylate (PMMA) has deficiencies in mechanical properties, so a reinforcing agent, chitosan, is required. Chitosan can improve properties of PMMA. This experimental laboratory study was aimed to determine effects of 5, 6, 7, 8, and 9% chitosan addition on PMMA denture base on surface roughness and against Candida albicans growth. Methods: A total of 42 samples (12 x 12 x 3 mm) for surface roughness and 30 samples (10 x 10 x 1 mm) for Candida albicans were divided into 6 groups (control, 5, 6, 7, 8, and 9%). The chitosan was mixed with PMMA and cured to create acrylic plates. Samples were contaminated with Candida albicans and the remaining colonies were measured by colony counters. Surface roughness value were analyzed by One-way ANOVA and LSD meanwhile Candida albicans growth were analyzed by Kruskal-Wallis and Mann-Whitney. Results: The mean value of surface roughness on control group was 0.105 ± 0.006; 5, 6, 7, 8, and 9% was 0.123 ± 0.006; 0.137 ± 0.005; 0.147 ± 0.004; 0.158 ± 0.005; 0.176 ± 0.007. The mean value of the number of Candida albicans on control group was 250.60 ± 6.88; 5%, 6%,7%,8%,9% at 112.60 ± 4.78; 95.00 ± 2.00; 84.40 ± 3.29; 5.20 ± 1.64; 0.60 ± 0.90 with p value = 0.0001 (p<0.05). Conclusion: Addition of 5% chitosan can be used as reinforcement material of PMMA with surface roughness value not exceeding 0.2 μm so that it was clinically accepted. Addition of 9% chitosan was effective in reducing adherence of Candida albicans.

Key word

heat polymerized acrylic resin, reinforcement, chitosan, surface roughness, candida albicans



Keywords


resin akrilik polimerisasi panas, bahan penguat, kitosan, kekasaran permukaan, candida albicans

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References


Hartini VO, Widodo HB, Purnama RB, Logamarta SW, Imam DNA. Absorption of heat cured acrylic resin reinforced with rice husk nanoceluloce (Oryza Sativa

L.). J Dentomax Sci. 2021; 3: 184.

Oetami S, Handayani M. Gigi tiruan lengkap resin akrilik pada kasus full edentulous. JIKG. 2021; 4(2): 54. DOI: 10.23917/jikg.v4i2.15967

Rahmawati SJ, Logamarta SW, Satrio R. Penambahan nanoselulosa sekam padi terhadap kekasaran permukaan basis gigi tiruan resin akrilik polimerisasi panas.

Insis Dent J. 2021; 10(2):45-50.

Gopi N, Venkatraman J. Mechanical properties and surface roughness of chitosan reinforced heat polymerized denture base resin. J Prosthodont Res. 2022;

(1): 101-2. DOI: 10.2186/jpr.JPR_D_20_00257

Rumengan IFM, Suptijah P, Salindeho N, Wullur S, Luntungan AH. Nanokitosan dari sisik ikan: aplikasinya sebagai pengemas produk perikanan. Manado: Lembaga Penelitian dan Pengabdian Kepada Masyarakat, 2018: 7-14, 16-8, 35, 52, 59-65, 90-2, 95-6.

Adiana ID, Syafiar L. Penggunaan kitosan sebagai biomaterial di kedokteran gigi. Dentika: Dent Jl. 2014; 18(2): 190-193.

Amer ZJA, Ahmed JK, Abbas SF. Chitosan/PMMA bioblend for drug release applications. Int J Engineering Tech. 2014; 4(5): 318-24.

Lubis MDO, Putranti DT. Pengaruh penambahan aluminium oksida pada bahan basis gigi tiruan resin akrilik polimerisasi panas terhadap kekerasan dan kekasaran permukaan. B-Dent. 2019; 6(1): 2-3. DOI:10.33854/jbd.v6i1.202

Fadriyanti, O, Putri FI, Surya LS. Perbedaan kekasaran permukaan resin akrilik yang direndam dalam larutan sodium hipoklorit dan ekstrak jamur Endofit

Aspergillus Sp (Akar Rhizophora Mucronata). B-Dent: J Ked Gigi Univ Baiturrahmah. 2018; 5(2): 153-161.

Bajunaid SO. How effective are antimicrobial agents on preventing the adhesion of Candida albicans to denture ase acrylic resin materials? A systematic review.

Polymers. 2022; 14(908): 1-2. DOI: 10.3390/polym14050908

Gondim BLC, Castellano LRC, Castro RD, Machado G, Carlo HL, Valenca AMG, et al. Effect of chitosan nanoparticles on the inhibition of Candida spp. biofilm on denture base surface. Archieve of Oral Biology. 2018; 94: 99-107. DOI: 10.1016/j.archoralbio.2018.07.004

Ismiyati T, Alhasyimi AA. The effect of chitosan addition in acrylic resin matrix towards the residual monomers and impact strength. Res J Pharm Tech. 2021;

(4): 2280-5. DOI: 10.52711/0974-360X.2021.00403

Sadeghi AZ, Falahati M, Sayah AS, Ashrafi KM, Rostam KF, Bahador A. The effect of nanochitosans particles on Candida biofilm formation. Curr Med Mycol

; 2(2): 29-30, 32. DOI: 10.18869/acadpub.cmm.2.2.1

Ismiyati T, Siswomihardjo W, Soesatyo MHNE, dkk. Campuran kitosan dengan resin akrilik sebagai bahan gigi tiruan penghambat Candida albicans. Maj Ked Gi

Ind 2017; 3(3): 139-41, 143-4. DOI: 10.22146/majkedgiind.23721

Ritonga PWU, Nurdiana. Disinfection effect of 10 % ricinus comunis oil on Candida albicans counts of heat polymerized acrylic resin. Dentika Dental Journal.

; 24(1): 9-10. DOI: 10.32734/dentika.v24i1.5615

Manappalil JJ. Basic dental material 4th ed. India :Jaypee Brothers Medical Publisher, 2016 381-384, 391-9, 404-8.

Syafrinani, S., & Setiawan, Y. Perbedaan kekasaran permukaan basis resin akrilik polimerisasi panas menggunakan bahan pumis, cangkang telur dan pasta gigi sebagai bahan poles. J Ilmiah PANNMED. 2017; 12(2): 200-3.

Wardojo CV, Teguh PB, Rochyani L. The difference of surface roughness of heat cured acrylic resin after brushing with 30% and 60% lemongrass extract in pasta. DENTA. 2019; 13(1): 17-24. DOI: 10.30649/denta.v13i1.178

Fransisca W, Nasution ID. Pengaruh penambahan serat kaca dan serat poliester terhadap kekuatan impak bahan basis gigitiruan resin akrilik polimerisasi panas.

B-Dent: J Ked Gi Univ Baiturrah. 2015; 2(1): 16-22. DOI:10.33854/JBDjbd.10

Brasselet C, Pierre G, Dubessay P, Dols-Lafargue M, Coulon J, Maupeu J, et al. Modification of chitosan for the generation of functional derivatives. Appl. Sci.

; 9(1321): 1-33. DOI: 10.3390/app9071321

Evelyna A, Sutanto D, Tiffany E. Chitosan 2% effect on prohibiting the growth of Candida albicans on heat cured acrylic resin. JMKG. 2017; 6(2): 19, 22-3.

Minh NC, Hoa NV, Trung TS. Preparation, properties, and application of low-molecular-weight chitosan in: handbook of chitin and chitosan. Vietnam: Elsevier,

: 454-63.17. DOI: 10.1016/b978-0-12-817970-3.00015-8

Agustina S, Swantara IMD, Suartha IN. Isolasi kitin, karakterisasi, dan sintesis kitosan dari kulit udang. J Kimia, 2015; 9(2): 271-8.

Sosiati H, Al-Giffari F, Adil FA, Kamiel BP, Adi RK, Yusuf Y. The properties of kenaf/carbon/PMMA hybrid composites by adding chitosan nano and microparticles.

Materials Today: Proceedings 2022; 66: 2908-13. DOI: 10.1016/j.matpr.2022.06.556

Lo WH, Deng FS, Chang CJ, Lin CH. Synergistic antifungal activity of chitosan with fluconazole against Candida albicans, Candida tropicalis, and fluconazole- resistant strains. Molecules. 2020; 25(5114): 1-13. DOI: 10.3390/molecules25215114




DOI: https://doi.org/10.24198/jkg.v35i3.47990

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