Perbedaan efek pemberian gel kombinasi ekstrak Camellia sinensis dan kitosan terhadap jumlah sel neutrofil pasca ekstraksi gigi tikus Wistar: eksperimental laboratoris

Nyoman Ayu Anggayanti; Eka Pramudita Ramadhany; I Gusti Ayu Putu Diah Suryani

doi:10.24198/jkg.v37i1.59224

Perbedaan efek pemberian gel kombinasi ekstrak Camellia sinensis dan kitosan terhadap jumlah sel neutrofil pasca ekstraksi gigi tikus Wistar: eksperimental laboratoris

Nyoman Ayu Anggayanti, Eka Pramudita Ramadhany, I Gusti Ayu Putu Diah Suryani

Abstract

Pendahuluan: Neutrofil berperan penting dalam mengatur peradangan selama penyembuhan luka. Camellia sinensis (teh hijau) terbukti mengandung Epigallocatechin Gallate (EGCG) yang memiliki aktivitas anti inflamasi. Kitosan dengan kemampuannya menjaga stabilitas EGCG, berpotensi untuk meningkatkan efektivitas EGCG dalam mengurangi inflamasi. Penelitian ini bertujuan untuk menganalisis perbedaan efek pemberian gel ekstrak Camellia sinensis yang dikombinasikan dengan kitosan terhadap jumlah neutrofil sebagai indikator utama fase inflamasi pada tikus Wistar pasca ekstraksi gigi. Metode: Gigi insisivus mandibula kiri pada tikus diekstraksi setelah dilakukan anestesi. Soket pada kelompok perlakuan diberi gel kombinasi ekstrak Camellia sinensis dan kitosan, sedangkan kelompok kontrol tidak diberikan intervensi. Pemeriksaan histopatologi soket dilakukan untuk menilai keberadaan neutrofil pada hari pertama, ketiga, kelima, dan ketujuh pasca perlakuan. Data yang diperoleh dianalisis menggunakan uji Kruskal-Wallis dan Mann-Whitney berdasarkan karakteristik distribusi. Hasil: Jumlah neutrofil pada kelompok perlakuan mengalami penurunan yang signifikan disetiap hari pengamatan dibandingkan kelompok kontrol yang menunjukan proses penyembuhan yang lebih baik dengan tidak adanya inflamasi persisten (p=0.000, p<0.05). Simpulan: Kombinasi ekstrak Camellia sinensis dan kitosan berefek terhadap penurunan jumlah neutrofil pasca ekstraksi gigi tikus Wistar.

The effect of gel Camellia sinensis extract in combination with chitosan on neutrophils count of Wistar rats after tooth extraction: A Laboratory Experimental Study

Introduction: Neutrophils play a key role in regulating inflammation during wound healing. Camellia sinensis has been proven to contain Epigallocatechin Gallate (EGCG), which exhibits anti-inflammatory activity. Chitosan with its ability to maintain EGCG stability, offers the potential to enhance the effectiveness of EGCG in reducing inflammation. This research aims to analyze the effect of extract gel Camellia sinensis combined with chitosan on the number of neutrophils as the main indicator of the inflammatory phase in Wistar rats after tooth extraction. Methods: The left mandibular incisor was extracted after the rats were anesthetized. The socket in the treatment group was treated with gel combining Camellia sinensis extract and chitosan, while the control group did receive any intervention. A histopathological examination of the socket was conducted to assess the presence of neutrophil on days one, three, five, and seven after treatment. The obtained data were analyzed using Kruskal-Wallis and Mann-Whitney tests based on the distribution characteristics. Results: The number of neutrophils in the treatment group decreased significantly on each day of observation compared to the control group, indicating a more advanced healing process with the absence of persistent inflammation (p=0.000, p<0.05). Conclusion: The combination of Camellia sinensis extract and chitosan had an effect on reducing the number of neutrophils in Wistar rats after tooth extraction.

Keywords

Pencabutan gigi, neutrofil, Camellia sinensis, kitosan

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DOI: https://doi.org/10.24198/jkg.v37i1.59224