Quality and Longevity of Local Ram’s Sexed Sperm with Albumin Colums

Nurcholidah Solihati, Siti Darodjah Rasad, Annisaa Yusrina, Kikin Winangun, Toha .

Abstract


Sperm sexing applications can support the effectiveness of artificial insemination (AI) programs in sheep. The albumin column method is one method of sperm sexing that is relatively easy and inexpensive compared to other methods, but research needs to be done to obtain a method that results in the quality and longevity of sexed sperm that is feasible to use for AI programs, one of which is by searching for incubation or sexing time. This study used 10 ejaculate semen from two local sheep around 2-3 years old. We used bovine serume albumin (BSA) as the albumin column with two fractions which is 5% in the upper fraction and 10% in the lower fraction. The treatment given was three lengths of incubation/sexing time (45, 60 and 75 minutes) which were repeated 10 times. Parameters consist of the quality of semen (motility, abnormalities, intact plasma membrane, intact acrosome cup) and longevity. Data were analyzed by analysis of variance and Duncan's advanced test. The results showed that the length of sexing time had a very significant effect on the quality of semen, but the length of incubation/sexing time up to 75 minutes still produced the quality of semen that was qualify for AI. The result also showed that incubation/sexing time does not affect the longevity of sexed sperm. It was concluded that the quality of sexed sperm of local ram that sexing with albumin colums fulfilled the quality for artificial insemination and the incubation/sexing time for 45-75 minutes can be used to obtain the qualify sexed sperm for artificial insemination with chilled semen.

Keywords


sperm sexing, albumin colums, incubation time

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References


Agarwal, A., Saleh, R.A. & Bedalwy, M. A. 2003. Role of Reactive Oxygen Species in The Phatophysiology of Human Reproduction. Fertil and Steril. 79:829-843.

Aini, A.N., Setiadi, M.A. & Karja, N.W.K. 2016. Kemampuan Fertilisasi Spermatozoa Sexing dan Perkembangan Awal Embrio Secara In Vitro pada Sapi. Jurnal Sain Veteriner 34(2):225-232

Ariantie, O. S., Yusuf T. L., Sajuthi, D, & Arifiantini, R.I. 2013. Pengaruh Krioprotektan Gliserol dan Dimethilformamida dalam Pembekuan Semen Kambing Peranakan Etawah Menggunakan Pengencer Tris Modifikasi. Jurnal Ilmu Ternak dan Veteriner 18 (4): 239-250.

Baqir, M., Fakhrildin M. R. & Kouty, B. K. 2009. Outcomes of Sperm Parameters, Hypo-Osmotic Swelling Test and Intra-Uterine Insemination for Varicocelic and Non-Varicocelic Infertile Patients. Journal Dohuk University, Vol. 12. No. 1. p. 301-306.

Bearden, H. J. & Fuquay, J. W.. 2000. The Male Reproduction System. In: Applied Animal Reproduction. 5th Edition. Prentice. P. 29.

Bohlooli, S., Cedden, F., Zozoglu, S. & Pishjang, J. 2012. Correlation Between Conventional Sperm Assay Parameters in Cryopreserved Ram Semen. Journal Ann. Biol Res. 3 : 884-889.

Burroughs, C. A. 2011. Removing Seminal Plasma Improves Sex-Sorting of Bovine Sperm. Thesis. Department of Biomedical Sciences. Colorado State University. Colorado. P. 17.

Afriani, T., Udin, Z. , Jaswandi & Asmairicen, S. 2011. Pengaruh Waktu Pelapisan Spermatozoa Sapi pada Media TALP yang Disuplementasi Bovine Serum Albumin (BSA) Terhadap Jenis Kelamin Embrio In Vitro. Jurnal Peternakan Indonesia. Vol. 13. No. 2. Hlm. 141-142.

Diliyana, D. F., Susilawati, T. & Rahayu, S. 2014. Keutuhan Membran Spermatozoa Disekuensing Sentrifugasi Gradient Densitas Percoll Berpengencer Andromed dan CEP-2 yang Ditambahkan Kuning Telur. Jurnal Veteriner Vol 15. No 1. page 23-30.

Garner DL. Flow cytometric sexing of mammalian sperm. 2006. Theriogenology. 65:943–57.

Hadi, S. & Al-Timimi, I. H. 2013. Separation of Y-Chromosome Bearing Ram’s Sperms using an Albumin Gradient Technique and Identification of Embryos by PCR. AL-Qadisiya Journal of Vet. Med.Sci. Unive. of Baghdad. Vol. 12. No. 1. P. 147-148.

Henkel Ralf, P.D. 2004. Spermatozoa Functional Assays. Fertilization Reproduction Magazine. 1-3

Ihsan, N. M. 2009. Bioteknologi Reproduksi Ternak. Universitas Brawijaya. Malang.

Iwazaki, A. & C. Gagnon. 1992. Formation of Reactive Oxygen Species in Spermatozoa of Infertil Patient. J. Fertil. Steril. 57: 408-416.

Kaiin, E. M., Gunawan, M., Afiati, F., Said, S., & Tappa, B. 2013. Production of Frozen Sexing Sperm Separated with BSA Colums Method with Standarized on Artificial Insemination Center. Proceedings International Conference on Biotechnology. Bogor. 67-72.

Kaiin, E. M., Gunawan, M. & Said, S. 2015. Aplikasi Inseminasi Buatan dengan Sperma Sexing dalam Meningkatkan Produktivitas Sapi di Peternakan Rakyat. Prosiding Seminar Nasional. Pusat Penelitian Bioteknologi LIPI. Vol. 1 No. 1 Maret 2015 [93-96].

Kaiin, E.M. & Gunawan, M.2017. Kualitas Sperma Hasil Sexing setelah Kapasitasi secara In Vitro. Pros Sem Nas Masy Biodiv Indon. Vol 3(3): 466-470

Manjunant, P., Therien, I. & Souberyrand, I. 1997. Major Protein of Bovine Seminal Plasma Modulate Sperm Capacitation by High Density Lipoprotein. Biology. Reproduction. 57(5):1080-1088.

Peippo J, Raty, M., Korhomen, K., Eronen, M., Kananen, K., Turme, T., Halmekyto, M. & Maki-Tanila, A. 2010. Impact of in vitro fertilization of bovine oocytes with sex-sorted frozen-thawed spermatozoa on developmental kinetics, quality and sex ratio of developing embryos. Zygote 2010;1:1–10.

Sharma, M., Sigh, M., Kapoor, S. & Jaisal S. 2012. Inter Relationship Between Some Routine Semen Evaluation Parameters in Jersey X Local Hill Cattle Crossbred Bulls. Open Veteriner Journal. 2 : 26-31.

Sianturi, R. G., Purwantara, B., Supriatna, I., Amrozi & Situmorang, P. 2012. Pengaruh Glutation dan Penggantian plasma Semen Kerbau dengan Plasma Semen Sapi Terhadap Kualitas Semen Beku Kerbau Rawa (Bubalus bubalis). JITV 17 (3): 169-178.

Situmorang, P., R.G. Sianturi, D.A. Kusumaningrum & Ross dan Maidaswar. 2013. Kelahiran Anak Sapi Perah Betina Hasil Inseminasi Buatan Menggunakan Sexed Spermatozoa yang Dipisahkan dengan Kolom Albumin Telur. Balai Inseminasi Buatan Lembang, Bandung.

Solihati, N., Rasad, S.D., Setiawan R. & Alvionita C. 2016. Quality and Viability of Javanese Local Ram Semen at Different Age. Proceeding International Seminar on Livestock Production and Veterinary Technology. 265 - 270.

Solihati, N, Rasad S.D. & Yusrina, A. 2017 Proc. International Seminar on Animal Production (Yogyakarta)) Vol 7 (Universitas Gadjah Mada, Yogyakarta)

Solihati, N., Rasad, S.D., Setiawan, R. & Nurjanah, S. 2018. Pengaruh Kadar Gliserol Terhadap Kualitas Semen Domba Lokal. Biodjati. Vol 3 (1):63-71

Sukmawati, E., Arifiantini, R. I. & Purwantara, B. 2014. Daya Tahan Spermatozoa terhadap Proses Pembekuan pada Berbagai Jenis Sapi Pejantan Unggul. Pascasarjana Biologi. Institut Pertannian Bogor, Bogor. JITV. Vol 19(3): 168-175.

Sunarti, T. Saili & Nafiu, L.O. 2016. Karakteristik Spermatozoa Sapi Bali Setelah Sexing Menggunakan Metode Kolom Albumin dengan Lama Waktu Sexing yang Berbeda. Fakultas Peternakan UHO, Kendari. Jitro 1(1): 65-76.

Susilowati, S. 2010. Efek Waktu Sentrifugasi Terhadap Motilitas, Daya Tahan Hidup, dan Tudung Akrosom Spermatozoa Kambing. Veterinaria Medika. Fakultas Kedokteran Hewan. Universitas Airlangga. Vol 3(1) :61-63.

Underwood SL, Bathgate, R, Pereira, DC, Castro A, Thomson PC, Maxwell WMC, & Evans G. 2010. Embryo production after in vitro fertilization with frozen-thawed, sex-sorted, re-frozen bull sperm. Theriogenology. 73:97




DOI: https://doi.org/10.24198/jit.v19i2.25632

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