Pengujian efektivitas berbagai jenis dan konsentrasi sitokinin terhadap multiplikasi tunas mikro pisang (Musa paradisiaca L.) secara In Vitro
Abstract
Perbanyakan tanaman pisang secara konvensional dengan menggunakan bonggol atau anakan (sucker) secara terus menerus dapat menurunkan kualitas dan memerlukan waktu lama serta meningkatkan terjadinya serangan penyakit. Oleh karena itu, suatu upaya untuk menghasilkan bibit yang memiliki kualitas baik dalam waktu yang cepat dapat menggunakan kultur in vitro. Percobaan ini bertujuan untuk memperoleh laju multiplikasi yang tinggi dengan penggunaan salah satu jenis dan konsentrasi sitokinin pada kultur in vitro tanaman pisang kepok kuning. Percobaan dilakukan pada bulan Oktober hingga Desember 2015 di Laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Rancangan percobaan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 8 perlakuan penggunaan jenis dan konsentrasi sitokinin yang berbeda (2 mg L-1 BAP, 4 mg L-1 BAP, 2 mg L-1 2-iP, 4 mg L-1 2-iP, 0,08 mg L-1 TDZ, 0,2 mg L-1 TDZ, 2 mg L-1 Kn, 4 mg L-1 Kn). Hasil percobaan menunjukkan bahwa 2 mg L-1 BAP merupakan jenis sitokinin yang mampu menghasilkan tingkat multiplikasi yang tinggi pada peubah persentase eksplan bertunas (%), dan tinggi tunas (cm).
Kata Kunci : efektivitas, kepok kuning, multiplikasi, pisang, sitokinin, tunas
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DOI: https://doi.org/10.24198/kultivasi.v15i2.11870
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