Multiplikasi tunas mikro pisang (Musa paradisiaca l.) ‘raja bulu’ secara in vitro pada berbagai jenis dan konsentrasi sitokinin
Abstract
Terbatasnya ketersediaan bibit pisang bermutu di Indonesia disebabkan oleh masih rendahnya jumlah dan kualitas bibit pisang yang dihasilkan melalui metode konvensional. Waktu yang dibutuhkan untuk mendapatkan bibit cukup lama, oleh karena itu digunakan suatu metode perbanyakan untuk menghasilkan bibit dalam waktu yang relatif singkat melalui metode kultur jaringan. Tujuan dari penelitian ini adalah untuk mendapatkan salah satu jenis dan konsentrasi sitokinin terbaik dalam meningkatkan laju multiplikasi tunas pisang raja bulu secara in vitro. Percobaan dilaksanakan dari bulan November 2016 sampai bulan Maret 2017 di Laboratorium Kultur Jaringan dan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran di Jatinangor, Sumedang. Rancangan Acak Lengkap (RAL) digunakan dalam penelitian ini dengan 13 perlakuan, 3 ulangan dan 2 sampel. Media Murashige and Skoog (MS) digunakan sebagai media dasar dengan kombinasi konsentrasi BAP (1; 1,5; 2 and 2,5 mg L-1), Thidiazuron (TDZ) (0,1; 0,3; 0,5 and 0,7 mg L-1) and Kinetin (1,5; 2; 2,5 and 3 mg L-1) Hasil penelitian menunjukkan bahwa TDZ 0,1 mg L-1 mampu meningkatkan jumlah tunas pisang 'Raja Bulu', oleh karena itu menunjukkan bahwa TDZ 0,1 mg L-1 merupakan jenis sitokinin yang potensial untuk proses multiplikasi tunas pada pisang 'Raja Bulu' secara in vitro.
Kata Kunci : BAP, In Vitro, Kinetin, Musa paradisiaca L., TDZ
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DOI: https://doi.org/10.24198/kultivasi.v16i3.14917
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