In vitro multiplication of strawberry in various types and concentrations of cytokinins and auxins

Erni Suminar; Vira Rahmawati; Sumadi Sumadi; Anne Nuraini

doi:10.24198/kultivasi.v23i1.42727

In vitro multiplication of strawberry in various types and concentrations of cytokinins and auxins

Erni Suminar, Vira Rahmawati, Sumadi Sumadi, Anne Nuraini

Abstract

Growth regulator is one of the important factors in the success of plant propagation by tissue culture. 6-Benzylaminopurine (BAP) is one of the most widely used cytokinin because it is more stable, less expensive and more effective. Thidiazuron (TDZ) can increase the action of other cytokinins, either exogenous cytokinin or endogenous cytokinin. 1-Naphthaleneacetic acid (NAA) belongs to the more stable auxin group, because it is not easily decomposed and oxidized by enzymes released by plants. This study aimed to obtain the best types and concentrations of BAP, TDZ, and NAA for the in vitro multiplication of strawberry explant. The experiment was conducted at the Seed Technology and Tissue Culture Laboratory, Faculty of Agriculture, Universitas Padjadjaran, from April to July 2022. The explants used were axillary shoots of strawberry var. Sweet Charlie is derived from plantlets that were propagated in vitro. The experimental design used was a completely randomized design with six treatments and four replications. The media used were Murashige dan Skoog (MS) with the addition of plant growth regulators (PGRs) in the form of BAP (0 ppm and 0,5 ppm), TDZ (0 ppm and 0,1 ppm), and NAA (0 ppm and 0,1 ppm). The application of different types and concentrations of BAP, TDZ, and NAA resulted in different effects of in vitro shoot multiplication of strawberry explant var. Sweet Charlie. Concentration of 0.1 ppm TDZ produced the highest number of shoots (5.38).

Keywords

BAP; TDZ; NAA; Strawberry; Tissue Culture

Full Text:

PDF

References

Angin YP, Purwaningrum Y, Asbur Y, Rahayu MS, Nurhayati. 2019. Utilization of secondary metabolite content produced by plants in biotic stress. Agriland, 7(1): 39-47.

Bimantara DS, Maghfoer MD, Barunawati N, Yenni Y, Siregar AS. 2018. Multiplication of strawberry meristem culture of the Earlibrite cultivar with the addition of BAP and Kinetin hormone concentrations. Jurnal Produksi Tanaman, 6(3): 432- 437.

Capelletti R, Sabbani S, Mezzetti B. 2016. The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars. Scientia Horticulturae, 207: 117-124.

Danial GH, Ibrahim DA, Omer MS. 2016. Response of running shoot tips of strawberry (Fragaria x ananasa) for in vitro propagation in Kurdistan Region of Iraq. International Journal of Environment, Agriculture and Biotechnology (IJEAB), 1(2): 164-169.

Guo B, Abbasi BH, Zeb A, Xu LL, Wei YH. 2011. Thidiazuron: A multi-dimensional plant growth regulator. African Journal of Biotechnology, 10(45): 8984-9000.

Haddadi F, Aziz MA, Saleh G, Rashid AA, Kamaladini H. 2010. Micropropagation of strawberry cv. camarosa: Prolific shoot regeneration from in vitro shoot tips using thidiazuron with 6-benzylaminopurine. HortScience, 45(3): 453–456.

Hardiyati T, Budisantoso I, Kamsinah, Suwito E. 2017. Perbanyakan Tanaman Anggrek Menggunakan Teknik Kultur in Vitro. Universitas Jenderal Soedirman. Purwokerto.

Kurnia IM. 2014. Plant Hormones. Available online in https://distan.bulelengkab.go.id/informasi/detail/artikel/hormon-tumbuhan-77. Accessed at 29 Januari 2022.

Kusmianto J. 2008. Effect of single thidiazuron and combination of thidiazuron and benzyl amino purine on shoot formation from Dendrobium antennatum Lindl leaf cuttings in vitro. Faculty of Mathematics and Natural Sciences. Universitas Indonesia.

Lestari E, Nurhidayati T, Nurfadillah S. 2013. Effect of 2,4-D and BAP concentrations on growth and development of Dendrobium laxiflorum J.J Smith seeds in vitro. Jurnal Sains dan Seni Pomits, 2(1): 2337-3520.

Ministry of Agriculture of the Republic of Indonesia. 2019. Get to know the varieties and benefits of strawberries in Indonesia. Available online in http://pustaka.setjen.pertanian.go.id/index-berita/mengenal-varietas-stroberi-di-indonesia. Accessed at 16 November 2021.

Murti RH, Debnath SC, Yeoung YR. 2012. Effect of high concentration of thidiazuron (TDZ) combined with 1H-indole-3-butanoic acid (IBA) on Albion strawberry (Fragaria × ananassa) cultivar plantlets induction. African J. Biotechnol., 11(81): 14696-14702.

Mutmainah S. 2016. Induction of Adventitious Shoots of Single Garlic (Allium sativum) by Adding BAP and NAA in Vitro. Undergraduate thesis. Universitas Islam Negeri Maulana Malik Ibrahim.

Raisya E, Sobarna DS, Nuraini A, Mubarok S, Suminar E, Akutsu M. 2020. In vitro multiplication of Tochiotome strawberry cultivar by adding types and concentrations of cytokinins for seed propagation. Jurnal Kultivasi, 19(3): 1189-1195.

Rantau DE, Wulandari DR, Ermayanti TM, Rudiyanto, Hapsari BW, Wulansari A, Maulana E, Firdaus HL. 2021. Growth and morphology of shoot culture of sempur (Dillenia philippinensis Rolfe) on MS-BAP-NAA media. Jurnal Penelitian Hutan Tanaman, 18(1): 65-78.

Research Institute for Citrus and Subtropical Fruit Crops. 2015. Production of Disease-Free Strawberry Seeds Through Meristem Culture. Available online in http://balitjestro.litbang.pertanian.go.id/produksi-bibit-stroberi-bebas-penyakit-melalui-kultur-meristem/. Accessed at 18 November 2021.

Sjahril R, Haring F, Riadi M, et al. 2016. Performance of NAA, 2iP, BAP and TDZ on callus multiplication, shoots initiation and growth for efficient plant regeneration system in chrysanthemum (Chrysanthemum morifolium Ramat.). International Journal of Agriculture System, 4(1): 52-61.

Statistics Indonesia. 2020. Fruit Crop Production 2020. Available online in https://www.bps.go.id/indicator/55/62/1/produksi-tanaman-buah-buahan.html. Accessed at 16 November 2021.

Sulasiah A, Tumilisar C, Lestaria T. 2015. Effect of auxin type and concentration on root induction in Dendrobium sp shoots in vitro. Bioma, 11(1): 56–66.

Wahyuni IN. 2016. Identification and pathogenicity of powdery mildew fungus on strawberry plants (Fragaria spp.). Doctoral Dissertation. Universitas Gadjah Mada.

Wahyuni A, Satria B, Zainal A. 2020. Agarwood callus induction with NAA and BAP in vitro. Jurnal Penelitian Agronomi, 22(1): 39-44.

Widiastuti A. 2015. Assistance to strawberry farmers in Serang Village, Karangreja, Purbalingga, Central Java in plant health management through KKN-PPM Gadjah Mada University: The need for healthy strawberry seeds and increasing farmer involvement in group activities. Jurnal Pengabdian Kepada Masyarakat , 1(1): 53-61.

Zulkarnain. 2009. Kultur Jaringan Tanaman, Solusi Perbanyakan Tanaman Budi Daya. PT Bumi Aksara: Jakarta.

DOI: https://doi.org/10.24198/kultivasi.v23i1.42727