Comparison between the electrical conductivity method and radicle emergence test as a rapid test of sorghum seed vigor

Nugraheni Nugraheni; Bambang Pujiasmanto; Samanhudi Samanhudi; Amalia Tetrani Sakya

doi:10.24198/kultivasi.v22i2.46547

Comparison between the electrical conductivity method and radicle emergence test as a rapid test of sorghum seed vigor

Nugraheni Nugraheni, Bambang Pujiasmanto, Samanhudi Samanhudi, Amalia Tetrani Sakya

Abstract

Sorghum (Sorghum bicolor L. Moench) belongs to multipurpose crops. All parts of sorghum can be used both as main products and derivative products; some derivative products produced from the sorghum plant consist of sugar, bioethanol, biomass, handicraft raw materials and starch. This study aims to determine the time required for testing the vigor and viability of sorghum seeds using the electrical conductivity and radicle emergence methods. The study was conducted in two experimental stages using a completely randomized design. The first experiment consisted of two factors: the number of sorghum seeds (40, 70 and 100 grains) and the volume of soaking water (75, 100, 125, and 150 ml). The second experiment consisted of two factors: research method (germination/control method, electrical conduction method, and radicle emergence method) and varieties (consisted of Numbu, Kawali, Suri 3, and Suri 4). The first experiment's results showed that the best electrical conductivity method on Suri 4 varieties were 40 seeds and 150 ml water volume. The electrical conductivity value is negatively correlated with Germination capacity, vigor index, growth of speed, maximum growth potential, and sprout growth rate. Time needed for Electrical Conductivity method in this study was three days. The germination period of 96 hours gives the best results on the radicle emergence of sorghum seed varieties. Vigor index, growth of speed, germination capacity, and maximum growth potential are positively correlated with the value of radicula emergence.

Keywords: Electrical Conductivity, Radicle emergence, Vigor, Viability, Germination

Keywords

Electrical Conductivity; Radicle emergence; Vigor; Viability; Germination

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References

Andini SN, Sari MF, Septina, Pradana OC. 2021. Uji konduktivitas benih pada beberapa genotipe mutan kedelai hitam generasi mutan ke tiga (M3). Planta Simbiosa, 3(2): 1-6.

Astuti F, Budiman C, Ilyas S. 2020. Pengembangan metode uji cepat vigor benih kedelai dengan pemunculan radikula. J. Agronomi Indonesia, 48(2): 135-141

Chhetri S. 2009. Identification of accelerated conditions for seed vigor test in rice (Oryza sativa L.). Thesis. Suranaree University of Technology. Thailand.

Dehnavi AR, Zahedi M, Ludwiczak A, Perez SC, Piernik A. 2020. Effect of salinity on seed germination and seedling development of sorghum (Sorghum bicolor (L) Moench) genotypes. J. Agronomy MDPI, 10(6): 1-15.

Demir I, Kuzucu CO, Ermis S, Memis N, Kadioglu N. 2022. Estimation of seed longevity in onion seed lots by a vigor test of radicle emergence test in artificial ageing conditions. J.Horticulturae, 8(11): 1-10

Dwipa I, SaswitaW. 2017. Pengujian hasil dan mutu benih beberapa varietas kedelai dengan variasi jumlah satuan panas panen. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia, 3(1): 16-22.

Fatonah K, Suliansyah I, Rozen N. 2017. Electrical conductivity for seed vigor test in soghum (Sorghum bicolor). Cell Biology and Development, 1(1): 6-12.

Fitriani, Amri Y, Bahri S, Nadilla F. 2021. Pengaruh bio-invigorisasi benih dan biofungisida dari ganoderma sp untuk meningkatkan ketahanan dan mutu benih padi gogo. Jurnal Agrotek Tropika, 9(2): 345–355.

Hartati P. 2019. Hubungan deteriorasi dengan umur simpan benih melalui penggunaan indikator pengujian viabilitas dan vigor pada benih wijen (Sesanum indicum L). Thesis. Universitas Sumatera Utara. Medan.

Ilyas S. 2012. Ilmu dan teknologi benih teori dan hasil- hasil penelitian. IPB Press. Bogor.

International Seed Testing Association (ISTA). 2011. Seed science and technology. Zurich. Switzerland.

International Seed Testing Association (ISTA). 2014. International rules for seed testing. Bassersdorf. Switzerland.

International Seed Testing Association (ISTA). 2021. International rules for seed testing. Bassersdorf. Switzerland.

Justice OL, Bass LN. 1990. Prinsip dan praktek penyimpanan benih (1st ed.). Rajawali. Jakarta.

Kartasapoetra AG. 2003. Teknologi benih pengolahan benih dan tuntunan praktikum (keempat). PT Rineka Cipta. Jakarta.

Khairani M, Rozen N, Swasti E. 2022. Uji daya hantar listrik untuk benih padi (Oryza sativa L). Jurnal Pertanian Agros, 24(2): 496-504.

Kusumawardana A. 2019. Pengujian mutu benih cabai (Capsicum annum) dengan metode uji pemunculan radikula dan uji tetrazolium. Thesis. Universitas Sebelas Maret. Surakarta.

Luo Y, Guan YJ, Huang YT, Li J, Li Z, Hu J. 2015. Single counts of radicle emergence provides an alternative method to test seed vigour in sweet com. Seed Science and Technology, 43(3): 519–525.

Matthews S, Khajeh HM. 2006. Mean germination time as indicator of emergence performance in soil of seed lots of maize (Zea mays). Seed Science And Technology, 34(2): 339–347.

Matthews S, Powell A. 2011. Towards automated single counts of radicle emergence to predict seed and seedling vigour. Seed Science, 142: 44-48.

Murwantini E. 2013. penggunaan uji konduktivitas sebagai uji vigor pada benih gandum (Triticum aestivum L). Thesis. Universitas Andalas. Padang.

Noeryanti IDS, Kartika AM, Eris R. 2022. Pengembangan metode uji pemunculan radikula sebagai metode uji cepat viabilitas dan vigor pada beberapa varietas benih kopi arabika (Coffea arabica L). Jurnal Pertanian Tirtayasa, 4(1): 439-456.

Pramono E, Kamal M, Setiawan K, Tantia MA. 2019. Pengaruh lama simpan dan suhu ruang penyimpanan pada kemunduran dan vigor benih sorghum (Sorghum bicolor L Moench) varietas samurai-1. J. Agrotek Tropika, 7(2): 383-389.

Pujiasmanto B. 2017. Dasar-dasar teknologi benih (1st ed.). CV Indotama. Solo.

Sadjad S. 1993. Dari benih kepada benih. Gramedia. Jakarta.

Subagio H, Suryawati. 2013. Wilayah penghasil dan ragam penggunaan sorgum di indonesia. Badan Penelitian dan Pengembangan Pertanian Kementerian Pertanian. Jakarta.

Suhartanto MR. 2021. The radicle emergence method can be a solution for providing seeds for farmers. IPB University. Bogor.

Susilowati SH, Saliem HP. 2013. Perdagangan sorgum di pasar dunia dan asia serta prospek pengembangannya di indonesia. Badan Penelitian dan Pengembangan Pertanian Kementerian Pertanian. Jakarta.

Vieira RD, TeKrony DM, Egli DB, Bruenning WP, Panobianco M. 2008. Temperature during soybean seed storage and the amount of electrolytes of soaked seeds solution. Sci Agric (Piracicaba, Braz), 65 (5): 496-501.

Widajati E, Murniati E, Endah PR, Tatiek K, Suhartanto MR, Qadir A. 2013. Dasar ilmu dan teknologi benih. Institut Pertanian Bogor Press. Bogor.

Widowati S, Luna P. 2022. Nutritional and functional properties of sorghum (Sorghum bicolor (L) Moench) based products and potential valorisation of sorghum bran. IOP Conf. Series. Earth and Environmental Science, 1024 (012031): 1-9.

Yukti AM, Murwantini E, Fadhilah S, Nugraheni. 2018. Pengujian mutu benih tanaman pangan dan hortikultura berdasarkan ista rules 2018. Balai Besar PPMB-TPH. Depok.

DOI: https://doi.org/10.24198/kultivasi.v22i2.46547