Growth response and yield performance of upland rice intercropped with legumes
Abstract
Intercropping patterns in upland rice must be carefully managed to prevent excessive competition among crops. This research aimed to determine the ideal proportion of upland rice–legume intercropped and legume types for rice growth and yield. The research was conducted in Mersi, East Purwokerto, Banyumas, Central Java, from April–August 2025. The study was arranged in a split-plot design consisting of a main factor in form of the proportion of upland rice to legumes (1:1, 2:1, and 3:1), and a sub-factor, i.e., legume types (peanuts, mung beans, and soybeans). The data were analyzed using Analysis of Variance and Tukey's HSD test at α = 5%. The results showed that the height of upland rice plants in intercropping was significantly higher than in sole cropping at 35 and 56 days after planting (DAP). The SPAD leaf greenness index of upland rice leaves at 70 DAP in sole cropping was significantly higher than in intercropping. Intercropping produced the insignificant number of stems, leaves, panicles, dry weight, number, and weight of grain per plant as upland rice in sole cropping. The number and weight of empty grains per plant, as well as upland rice productivity in sole cropping, were significantly higher than in intercropping. Both factors did not significantly affect the growth and yield of upland rice under intercropping condition. Intercropping upland rice and peanuts at a 2:1 planting proportion resulted in a land equivalent ratio greater than 1, indicating that the system was productive and efficient despite a high competitive ratio.
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Afonso P, Castro I, Couto P, Leal F, Carnide V, Rosa E, Carvalho M. 2025. Root Phenotyping : A Contribution to Understanding Drought Stress Resilience in Grain Legumes. Agronomy, 15(4), 1–15.
Akanvou RK, Becker M, Bastiaans L, Kropff MJ. 2007. Morpho–physiological characteristics of Cover crops for analysis of upland rice production in relay intercropping systems. Sciences & Nature, 4(2), 205–216.
Aldiansyah S, Risna. 2023. Mapping of oldeman agro-climatic zone based on climate hazards group infrared precipitation with station database in Southeast Sulawesi. Ecotrophic, 17(2), 174–187.
Amanullah F, Ropalia, Lestari T. 2022. Budidaya kacang hijau ramah lingkungan. Seminar Nasional Penelitian Dan Pengabdian Pada Masyarakat 2022, 57–62.
Andrews DJ, Kassam AH. 1976. The Importance of Multiple Cropping in Increasing World Food Supplies. In American Society of Agronomy (Vol. 27). https://doi.org/10.2134/asaspecpub27.c1
Arifuddin A, Wangiyana W, Aryana I. 2019. Effect of relay-planting several legume species at various ages of rice on growth and yield of red rice grown together with legume crops under aerobic irrigation system. International Journal of Environment, Agriculture and Biotechnology, 4(6), 1704–1710.
Assefa E, Bitew Y. 2023. Enhancing the land use efficiency of low-land rice (Oryza sativa L.)—Grass pea (Lathyrus sativus L.) additive series relay intercropping in North-Western Ethiopia: A farmer’s indigenous knowledge. PLoS ONE, 18(7 July), 1–17. https://doi.org/10.1371/journal.pone.0281410
Aziez AF, Prasetyo A. 2022. Root growth response of soybean (Glycine max L.) under water deficit. Journal of Biodiversity and Biotechnology, 2(2), 63–69.
Aziz A, Basri AB, Nasir A, Pesireron M. 2010. Uji multilokasi 6 varietas padi gogo di Provinsi NAD. Prosiding Seminar Nasional: Mewujudkan Kedaulatan Pangan Pada Lahan, 27, 338–345.
Beets WC. 2019. Multiple cropping and tropical farming systems. CRC Press.
Benu FL, Lawa Y, Neolaka YAB. 2023. Mini Review: Peran biofertilizer pada pertanian lahan kering. Jurnal Beta Kimia, 3(1), 40–49. https://doi.org/10.35508/jbk.v3i1.11656
BPS. 2023. Harvested Area, Production and Yield Rate of Dryland Paddy, 2023. BPS Kabupaten Banyumas. https://banyumaskab.bps.go.id/id/statistics-table/1/NjM1IzE%3D/luas-panen-produksi-dan-rata-rata-produksi-padi-ladang-tahun-2023.html
BPS Kabupaten Banyumas. 2026. Kabupaten Banyumas Dalam Angka 2026 Volume 51. https://banyumaskab.bps.go.id/id/publication/2026/02/27/4e4f855322bf9f80022acafb/kabupaten-banyumas-dalam-angka-2026.html
Bukovsky-Reyes S, Isaac ME, Blesh J. 2019. Effects of intercropping and soil properties on root functional traits of cover crops. Agriculture, Ecosystems and Environment, 285(106614), 1–10. https://doi.org/10.1016/j.agee.2019.106614
Cavite HJM, Mactal AG, Evangelista EV, Cruz JA. 2021. Growth and yield response of upland rice to application of plant growth-promoting rhizobacteria. Journal of Plant Growth Regulation, 40(2), 494–508. https://doi.org/10.1007/s00344-020-10114-3
Deb D, Dutta S. 2022. The robustness of land equivalent ratio as a measure of yield advantage of multi-crop systems over monocultures. Experimental Results, 3, 1–13. https://doi.org/10.1017/exp.2021.33
Destiningsih R. 2016. Analisis Komoditas Unggulan Pangan Kabupaten Banyumas. Jurnal REP, 1(1), 35–48. https://doi.org/10.31002/rep.v1i1.51
Dharmawangsa L, Nujanah U, Pujiwati H, Setyowati N, Prasetyo P. 2020. Nilai kesetaraan lahan dan hasil jagung manis tumpangsari dengan kacang-kacangan di pertanian organik. Seminar Nasional Lahan Suboptimal, 1, 224–236.
Dong J, Shen X, Li Q, Xue Z, Hou X, Miao H, Ning H. 2024. Irrigation and fertilization scheduling for peanut cultivation under mulched drip irrigation in a desert-oasis area. Plants (Basel, Switzerland), 13(1), 144. https://doi.org/10.3390/plants13010144
Elsayed S, El-Hendawy S, Elsherbiny O, Okasha AM, Elmetwalli AH, Elwakeel AE, Memon MS, Ibrahim MEM, Ibrahim HH. 2023. Estimating chlorophyll content, production, and quality of sugar beet under various nitrogen levels using machine learning models and novel spectral indices. Agronomy, 13(11), 2743.
Fang Y. Qiu X, Guo T, Wang Y, Cheng T, Zhu Y, Chen Q, Cao W, Yao X, Niu Q, Hu Y, Gui L. 2020. An automatic method for counting wheat tiller number in the field with terrestrial LiDAR. Plant Methods, 16(1), 1–14. https://doi.org/10.1186/s13007-020-00672-8
Feng, L, Raza MA, Chen Y, Khalid MH Bin, Meraj TA, Ahsan F, Fan Y, Du J, Wu X, Song C, Liu C, Bawa G, Zhang Z, Yuan S, Yang F,Yang W. 2019. Narrow-wide row planting pattern improves the light environment and seed yields of intercrop species in relay intercropping system. PLoS ONE, 14(2), 1–19. https://doi.org/10.1371/journal.pone.0212885
Galo E. 2025. Performance of upland rice intercropped legumes and level of nitrogen fertilizer. International Journal of Agriculture and Biosciences 2025. 14(4), 740–747.
Gelaye Y, Li J, Luo H. 2025. Journal of Genetic Engineering and Biotechnology Exploring the role of Peanut ( Arachis hypogaea L .) root architecture in enhancing adaptation to climate change for sustainable agriculture and resilient crop production : A review. Journal of Genetic Engineering and Biotechnology, 23(3), 1–12. https://doi.org/10.1016/j.jgeb.2025.100535
Gollin D, Udry C. 2021. Heterogeneity, measurement error, and misallocation: Evidence from african agriculture. Journal of Political Economy, 129(1), 1–80. https://doi.org/10.1086/711369
Gunasekaran A, Seshadri G, Ramasamy S, Muthurajan R, Karuppasamy KS. 2023. Identification of newer stable genetic sources for high grain number per panicle and understanding the gene action for important panicle traits in rice. Plants, 12(2), 1–18. https://doi.org/10.3390/plants12020250
Harsono A, Harnowo D, Ginting E, Elisabeth DAA. 2021. Soybean in Indonesia: current status, challenges and opportunities to achieve self-sufficiency. In J. C. Jimenez-Lopez & A. Clemente (Eds.), Legumes Research - Volume 1. IntechOpen. https://doi.org/10.5772/intechopen.101264
Hawalid H. 2019. Respon pertumbuhan dan produksi tanaman kacang tanah (Arachis hypogaea L.) pada pemberian takaran pupuk organik cair limbah tahu dan jarak tanam yang berbeda. Klorofil, xiv(2), 78–82.
Heryani N, Rejekiningrum P. 2020. Pengembangan pertanian lahan kering iklim kering melalui implementasi panca kelola lahan. Jurnal Sumberdaya Lahan, 13(2), 63–71. https://doi.org/10.21082/jsdl.v13n2.2019.63-71
Hilty J, Muller B, Pantin F, Leuzinger S. 2021. Plant growth: the what, the how, and the why. New Phytologist, 232(1), 25–41. https://doi.org/10.1111/nph.17610
Homulle Z, George, TS, Karley AJ. 2022. Root traits with team benefits : understanding belowground interactions in intercropping systems. Plant and Soil, 417, 1–26. https://doi.org/10.1007/s11104-021-05165-8
Idaryani, Warda, Dahamarudin L. 2017. Karakter agronomis dan hasil beberapa varietas unggul padi pada lahan kering di Kabupaten Jeneponto. Prosiding Seminar Nasional: Mewujudkan Kedaulatan Pangan Pada Lahan Sub Optimal Melalui Inovasi Teknologi Pertanian Spesifik Lokasi, 202–207.
IP2TP. 2000. Budidaya Kacang Tanah di Lahan Kering. Instalasi Penelitian dan Pengkajian Teknologi Pertanian Mataram, Lembar Informasi Pertanian (Liptan) IP2TP Mataram No. 09/Liptann/2000, 2–4.
Iwuagwu C, Umechuruba C, Ononuju C, Nwogbaga A, Salaudeen M, Onejeme F. 2019. Effect of intercropping rice with maize and soybeans on disease incidence, severity and yield of rice in Abia State. International Journal of Agricultural Science. 4, 23–28. http://iaras.org/iaras/journals/ijas
Jarrar H, El-Keblawy A, Ghenai C, Abhilash PC, Bundela AK, Abideen Z, Sheteiwy MS. 2023. Seed enhancement technologies for sustainable dryland restoration: Coating and scarification. Science of The Total Environment, 904, 150–166. https://doi.org/https://doi.org/10.1016/j.scitotenv.2023.166150
Kalaitzidis A, Kadoglidou K, Mylonas I, Ghoghoberidze S, Ninou E, Katsantonis D. 2025. Investigating the impact of tillering on yield and yield-related traits in European rice cultivars. Agriculture; Basel, 15(6).
Karunarathna B, Maduwanthi AKMRB. 2022. Competition indices used to evaluate the agronomic and monetary advantage in intercropping: A review. AGRIEAST: Journal of Agricultural Sciences, 16(1), 25–40. https://orcid.org/0000-0002-3773-9402 25
Kebede E. 2021. Contribution, utilization, and improvement of legumes-driven biological nitrogen fixation in agricultural systems. Frontiers in Sustainable Food Systems, 5, 1–18. https://doi.org/10.3389/fsufs.2021.767998
Khanal U, Stott KJ, Armstrong R, Nuttall JG, Henry F, Christy BP, Mitchell M, Riffkin PA, Wallace AJ, Mccaskill M, Thayalakumaran T, Leary GJO. 2021. Intercropping — evaluating the advantages to broadacre systems. Agriculture, 11(453), 1–20. https://doi.org/https:// doi.org/10.3390/agriculture11050453
Khasanah N, van Noordwijk M, Slingerland M, Sofiyudin M, Stomph D, Migeon AF, Hairiah K. 2020. Oil palm agroforestry can achieve economic and environmental gains as indicated by multifunctional land equivalent ratios. Frontiers in Sustainable Food Systems, 3(January), 1–13. https://doi.org/10.3389/fsufs.2019.00122
Khatun M, Sarkar S, Era FM, Islam AKMM, Anwar MP, Fahad S, Datta R, Islam AKMA. 2021. Drought stress in grain legumes: effects, tolerance mechanisms and management. Agronomy, 11(12). https://doi.org/10.3390/agronomy11122374
Kokkini M, Gazoulis I, Danaskos M, Kontogeorgou V, Kanatas P, Travlos I. 2025. Enhancing ecosystem services in agriculture: the special role of legume intercropping. Frontiers in Sustainable Food Systems, 9, 1547879.
Kumar A, Taparia M, Madapu A, Rajalakshmi P, Marathi B, Desai UB. 2020. Discrimination of filled and unfilled grains of rice panicles using thermal and RGB images. Journal of Cereal Science, 95, 1–9. https://doi.org/10.1016/j.jcs.2020.103037
Liu H, Gao X, Li C, Cai Y, Song X, Zhao X. 2025. Intercropping increases plant water availability and water use efficiency: A synthesis. Agriculture, Ecosystems & Environment, 379, 109360. https://doi.org/https://doi.org/10.1016/j.agee.2024.109360
Lu S, Song Z, Chen W, Qian T, Zhang Y, Chen M, Li G. 2021. Counting dense leaves under natural environments via an improved deep-learning-based object detection algorithm. Agriculture (Switzerland), 11(10), 1–16. https://doi.org/10.3390/agriculture11101003
Luo D, Lin H. 2025. Evaluation of drought-tolerant legume varieties under rainfed conditions. Field Crop, 8(3), 126–138. https://doi.org/10.5376/fc.2025.08.0013
Mead R, Willey RW. 1980. The Concept of a ‘Land Equivalent Ratio’ and Advantages in Yields from Intercropping. Experimental Agriculture, 16(3), 217–228.
Meirelles FC, Cavalcante AG, Gonzaga AR, Coelho AP, van der Werf W, Bastiaans L, Arf O, Lemos LB. 2024. Relative sowing time and spatial arrangement in upland rice/legume intercropping systems. International Journal of Plant Production, 18(2), 161–174. https://doi.org/10.1007/s42106-024-00294-3
Mishra P, Singh U, Pandey CM, Mishra P, Pandey G. 2019. Application of student’s t-test, analysis of variance, and covariance. Annals of Cardiac Anaesthesia, 22(4), 407–411. https://doi.org/10.4103/aca.aca_94_19
Nandhini DU, Somasundaram E. 2020. Intercropping – A substantial component in sustainable organic agriculture. Ind. J. Pure App. Biosci. 8(2), 133–143.
Neamatollahi E, Jahansuz MR, Mazaheri D, Bannayan M. 2013. Intercropping. In E. Lichtfouse (Ed.), Sustainable Agriculture Reviews, vol 12. (pp. 119–142). Springer Netherlands. https://doi.org/10.1007/978-94-007-5961-9_4
Oldeman RL. 1980. The agroclimatic classification of rice-growing environments in Indonesia. In World Meteorological Organization; International Rice Research Institute: Proceedings of a symposium on the agrometeorology of the rice crop (pp. 47–55).
Ozioma EM, Nwabunwanne OE, Ukaoba ME, Daniel OI. 2024. Substitutive effects of upland rice and soybean plant populations on the performance of the component crops in intercropping system. System, 12(8), 13.
Paiman, Ardiyanta, Kusumastuti CT, Masulili A, Yussof SF. 2023. A review on the advantages of Jajar Legowo planting system in rice (Oryza sativa L .) cultivation. Res. Crop, 24(3), 433–441. https://doi.org/10.31830/2348-7542.2023.ROC-966
Papong JR, Cagasan UA. 2020. Growth and yield performance of upland rice (Oryza sativa L . var . zambales ) intercropped with mungbean (Vigna radiata L .) and peanut (Arachis hypogaea L .).International Journal of Agriculture, Forestry and Life Sciences 1, 34–41.
Parida AK, Sekhar S, Panda BB, Sahu G. 2022. Effect of Panicle Morphology on Grain Filling and Rice Yield : Genetic Control and Molecular Regulation. Frontiers in Genetics, 13, 1–23. https://doi.org/10.3389/fgene.2022.876198
Peraturan Daerah Kabupaten Banyumas Nomor 12 Tahun 2024. 2024. Rencana perlindungan dan pengelolaan lingkungan hidup TAHUN 2024-2054. https://static.banyumaskab.go.id/jdih/file/Peraturan-Nomor-12-Tahun-2024-tentang-peraturan-daerah-kabupaten-banyumas-nomor-12-tahun-2024-tentang-rencana-perlindungan-dan-pengelolaan-lingkungan-hidup-tahun-2024-2054.pdf
Pokharel SS, Yu H, Fang W, Parajulee MN, Chen F. 2023. Intercropping cover crops for a vital ecosystem service: a review of the biocontrol of insect pests in tea agroecosystems. Plants, 12(12), 1–14. https://doi.org/10.3390/plants12122361
Rambe SSM, Calista I, Wulandari TN, Saragih A. 2024. Budidaya Tanaman Padi Gogo Terstandar. In Balai Penerapan Standar Instrumen Pertanian (BPSIP) Bengkulu.
Raza MA, Cui L, Qin R, Yang F, Yang W. 2020. Strip-width determines competitive strengths and grain yields of intercrop species in relay intercropping system. Scientific Reports, 10(1), 1–12. https://doi.org/10.1038/s41598-020-78719-y
Raza MA, Din AMU, Zhiqi W, Gul H, Ur Rehman S, Bukhari B, Haider I, Rahman MHU, Liang X, Luo S, El Sabagh A, Qin R, Zhongming M. 2023. Spatial differences influence nitrogen uptake, grain yield, and land-use advantage of wheat/soybean relay intercropping systems. Scientific Reports, 13(1), 1–15. https://doi.org/10.1038/s41598-023-43288-3
Ruillé M, Beillouin D. 2026. Ecological drivers of intercropping performance for enhanced global crop production. Npj Sustainable Agroculture, 4(8), 1–9. https://doi.org/https://doi.org/10.1038/s44264-025-00110-z Ecological
Saraswati E, Gunawati ES, Pudyantini A. 2006. Identifikasi komoditas unggulan sektor pertanian tanaman pangan di wilayah Kabupaten Banyumas. Eko-Regional, 1(2), 117–123.
Simanulang ZA. 2008. 75-Padi-Gogo-Varietas-Situ-Bagendit.
Singh CM, Singh P, Tiwari C, Purwar S, Kumar M, Pratap A, Singh S, Chugh V, Mishra AK. 2021. Improving drought tolerance in mungbean (Vigna radiata L . Wilczek): Morpho-physiological, biochemical and molecular perspectives. Agronomy, 11(1534), 1–20. https://doi.org/https://doi.org/10.3390/agronomy11081534
Sution, Nurdin M. 2015. Keragaan beberapa varietas unggul baru padi sawah irigasi semi teknis tanam pada musim kemarau. Balai Pengkajian Teknologi Pertanian Kalimantan Barat, 6(45), 250–255.
Thilakarathna MS, McElroy MS, Chapagain T, Papadopoulos YA, Raizada MN. 2016. Belowground nitrogen transfer from legumes to non-legumes under managed herbaceous cropping systems. A review. Agronomy for Sustainable Development, 36(4), 1–16. https://doi.org/10.1007/s13593-016-0396-4
Turnage G. 2022. Sampling submersed aquatic plant biomass: fresh vs. dry weight. In Geosystems Research Institute, Mississippi State University.
Wang W, Wang BZ, Zhang W, Li MY, Li JM, Ji SJ, Abrar M, Rehman MMU, Khan W, Tao HY, Sheteiwy MS, Wang WY, Xiong YC. 2026. Cereal-legume intercropping stabilizes yield and economic advantages under variable rainfall in semiarid rainfed environment. European Journal of Agronomy, 174, 127942. https://doi.org/https://doi.org/10.1016/j.eja.2025.127942
Wang X, Wu Z, Zhou Q, Wang X, Song S, Dong S. 2022. Physiological response of soybean plants to water deficit. Frontiers in Plant Science, 12(809692), 1–12. https://doi.org/10.3389/fpls.2021.809692
Wang X, Zou D, Li C, Zhou W, Li K, Tang Q, Zhu X, Li X, Cao L. 2024. Analysis of characteristics of rice tillering dynamics influenced by sowing dates based on DTM. Heliyon, 10(19).
Wang Y, Han X, Zhao X, Zhang Y. 2024. Grain yield and interspecific competition in an oat-common vetch intercropping system at varying sowing density. Plant Science, 15, 1–13. https://doi.org/10.3389/fpls.2024.1344110
Wang Z, Li Y, Su X, Tao S, Feng X, Wang Q, Xu X, Liu Y, Michaletz ST, Shrestha N, Larjavaara M, Enquist BJ. 2019. Patterns and ecological determinants of woody plant height in eastern Eurasia and its relation to primary productivity. Journal of Plant Ecology, 12(5), 791–803. https://doi.org/10.1093/jpe/rtz025
Wenham K, Williams A, Rossignol T, Collins M. 2019. Critical period of moisture vulnerability in mungbeans. GRDC. https://grdc.com.au/resources-and-publications/grdc-update-papers/tab-content/grdc-update-papers/2020/08/critical-period-of-moisture-vulnerability-in-mungbeans
Willey RW, Rao MR. 1980. A competitive ratio for quantifying competition between intercropS. Expl Agric, 16(104), 117–125. https://doi.org/https://doi.org/10.1017/S0014479700010802
Winazira A, Ilyas I, Sufardi S. 2021. Status dan kendala kesuburan tanah pada lahan tegalan dan kebun campuran di Kecamatan Blang Bintang Kabupaten Aceh Besar. Jurnal Ilmiah Mahasiswa Pertanian, 6(2), 79–87. https://doi.org/10.17969/jimfp.v6i2.16950
Wu L, Weston LA, Zhu S, Zhou X. 2023. Rhizosphere interactions: root exudates and the rhizosphere microbiome. In Frontiers in plant science, 14, 1–4. https://doi.org/10.3389/fpls.2023.1281010
Xi Y, Du YL, Wang D, Ren JY, Luo WY, Peng Q, Fang WY, Li FM. 2024. Wheat genetic progress in biomass allocation and yield components: A global perspective. Field Crops Research, 318, 109617. https://doi.org/https://doi.org/10.1016/j.fcr.2024.109617
Xu Y, Chu C, Yao S. 2021. The impact of high-temperature stress on rice: Challenges and solutions. Crop Journal, 9(5), 963–976. https://doi.org/10.1016/j.cj.2021.02.011
Yue B, Jin Y, Wu S, Tan J, Chen Y, Zhong H, Chen G, Deng Y. 2025. Research on SPAD inversion of rice leaves at a field scale based on machine vision and leaf segmentation techniques. Agriculture (Switzerland), 15(12), 1–24. https://doi.org/10.3390/agriculture15121270
Yue X, Hu Y, Zhang H, Schmidhalter U. 2020. Evaluation of both spad reading and spad index on estimating the plant nitrogen status of winter wheat. International Journal of Plant Production, 14(1), 67–75. https://doi.org/10.1007/s42106-019-00068-2
Zhang Y, Liang K, Zhu F, Zhong X, Lu Z, Chen Y, Pan J, Lu C, Huang J, Ye Q, Yin Y, Peng Y, Mo Z, Fu Y. 2024. Differential study on estimation models for indica rice leaf spad value and nitrogen concentration based on hyperspectral monitoring. Remote Sensing, 16(23), 1–16. https://doi.org/10.3390/rs16234604
Zhen X, Huo W, Sanz-Saez A, Miao Y, Chen CY, Batchelor WD. 2024. Drought-tolerant peanut (Arachis hypogaea L.) varieties can mitigate negative impacts of climate change on yield in the Southeastern U.S. Computers and Electronics in Agriculture, 224(109105). https://doi.org/https://doi.org/10.1016/j.compag.2024.109105
Zustovi R, Landschoot S, Dewitte K, Verlinden G, Dubey R, Maenhout S, Haesaert G. 2024. Intercropping indices evaluation on grain legume-small grain cereals mixture : a critical meta-analysis review. Agronomy for Sustainable Development, 44(5), 1–21. https://doi.org/10.1007/s13593-023-00934-4
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