Expression of fibroblast growth factor-2 after application of the Queen's crepe-myrtle leaf (Lagerstroemia speciosa) and aloe vera extract gel in the wound healing process of hyperglycemic

Fachrul Razi; Andri Hardianto; Lucky Riawan; Bambang Pontjo Priosoeryanto

doi:10.24198/pjd.vol33no1.21276

Expression of fibroblast growth factor-2 after application of the Queen's crepe-myrtle leaf (Lagerstroemia speciosa) and aloe vera extract gel in the wound healing process of hyperglycemic

Fachrul Razi, Andri Hardianto, Lucky Riawan, Bambang Pontjo Priosoeryanto

Abstract

Introduction: Fibroblast Growth Factor-2 (FGF-2) have a role in stimulating the proliferation and migration of various types of cells in the wound healing process. Hyperglycemic conditions can disrupt the wound healing process. Lagerstroemia speciosa are known to have antyphyerglycemic and antioxidant effects. Aloe vera is a plant that has been used for long time in topical treatment of wounds.This study was aimed to analyse the FGF-2 after application of Queen's crepe-myrtle leaf (Lagerstroemia speciosa) and aloe vera extracts gel in the wound healing process of hyperglycemia. Methods: A pure experimental study was conducted with simple random sampling. The sample was 27 experimental animals (CI 95%; power test 80%) of Sprague Dawley rat induced to have a hyperglycemic state using alloxan and had their palate injured. The immunohistochemical examination was performed to assess FGF-2 expression level. Samples were divided into three groups with a simple random sampling technique: nine of the rats received the Lagerstroemia speciosa extract gel application; nine rats received the aloe vera extract gel application on the injured palate; another nine rats in the control group. Examination of the FGF-2 expression level was performed on the third, seventh, and fourteenth observation days. Results: Overall, the control group had a significant difference with the Lagerstroemia speciosa and aloe vera extract gel group (p-value<0.05), however, the Lagerstroemia speciosa group did not have any significant difference with the aloe vera group (0.123>0.05). Conclusion: There was no difference in the amount of FGF-2 expressions on the wound healing process of the injured palatal tissue of Sprague-Dawley rats with hyperglycemic after application of the Lagerstroemia speciosa and aloe vera extract gel.

Keywords

Lagerstroemia speciosa; Queen's crepe-myrtle; aloe vera; fibroblast growth factor-2; wound healing; hyperglycemic

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References

Martin P, Nunan R. Cellular and molecular mechanisms of repair in acute and chronic wound healing. Br J Dermatol. 2015; 173(2): 370-8. DOI: 10.1111/bjd.13954

Marchianti ACN, Sakinah EN, Elfiah U, Putri NKS, Wahyuliswari DI, Maulana M, et al. Gel formulations of Merremia mammosa (Lour.) accelerated wound healing of the wound in diabetic rats. J Tradit Complement Med. 2019; 11(1): 38-45. DOI: 10.1016/j.jtcme.2019.12.002

Nunes QM, Li Y, Sun C, Kinnunen TK, Fernig DG. Fibroblast growth factors as tissue repair and regeneration therapeutics. Peer J. 2016; 4: e1535. DOI: 10.7717/peerj.1535

Edamura K, Takahashi Y, Fujii A, Masuhiro Y, Narita T, Seki M, et al. Recombinant canine basic fibroblast growth factor-induced differentiation of canine bone marrow mesenchymal stem cells into voltage and glutamate-responsive neuron-like cells. Regen Ther. 2020; 15: 121-128. DOI: 10.1016/j.reth.2020.07.005

Brunicardi FC, Andersen DK, Billiar TR, Dunn DL, Hunter JG, et al. Schwartz’s Principles of Surgery. 10th ed. New York: McGraw-Hill Education; 2015. p. 241-66.

Nilugal KC, Perumal K, Ugander RE, Chittor AI. Evaluation of wound healing activity of Piper betle leaves and stem extract in experimental Wistar rats. Am J Pharm Res. 2014; 4(3): 443-52.

Nurcahyanti ADR, Arieselia Z, Kurniawan SV, Sofyan F, Wink M. Revisiting Bungur (Lagerstroemia speciosa) from Indonesia as an antidiabetic agent, its mode of action, and phylogenetic position. Pharmacogn Rev. 2018; 12(23): 40-5. DOI: 10.4103/phrev.phrev_20_17

Hormozi M, Assaei R, Boroujeni MB. The effect of aloe vera on the expression of wound healing factors (TGFβ1 and bFGF) in mouse embryonic fibroblast cell: In vitro study. Biomed Pharmacother. 2017; 88: 610-6. DOI: 10.1016/j.biopha.2017.01.095

Radha MH, Laxmipriya NP. Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review. J Tradit Complement Med. 2015; 5(1): 21-6. DOI: 10.1016/j.jtcme.2014.10.006

Torregroza C, Feige K, Schneider L, Bunte S, Stroethoff M, Heinen A, et al. Influence of hyperglycemia on dexmedetomidine-induced cardioprotection in the isolated perfused rat heart. J Clin Med. 2020; 9(5): 1445. DOI: 10.3390/jcm9051445

Fedchenko N, Reifenrath J. Different approaches for interpretation and reporting of immunohistochemistry analysis result in the bone tissue-A review. Diagn Pathol. 2014; 9: 221. DOI: 10.1186/s13000-014-0221-9

Nilugal KC, Perumal K, Ugander RE, Chittor AI. Evaluation of wound healing activity of Piper betle leaves and stem extract in experimental Wistar rats. Am J Pharm Tech Res. 2014; 4(3): 443-52.

Pal M, Thareja D, Majee C. Lagerstroemia species: A review. Int J Pharm. 2016; 6(1): 95-8.

Chan EWC, Tan LN, Wong SK. Phytochemistry and pharmacology of Lagerstroemia speciosa: A natural remedy for diabetes. Int J Herb Med. 2014; 2(1): 81-7.

Hashemi SA, Madani SA, Abediankenari S. The review on properties of Aloe vera in healing of cutaneous wounds. BioMed Res Int. 2015; 2015: 714216. DOI: 10.1155/2015/714216

Mohamed EAK. Antidiabetic, antihypercholestermic and antioxidative effect of aloe vera gel extract in alloxan induced diabetic rats. Aust J Basic Appl Sci. 2011; 5(11): 1321-7.

Kayode OA. Effects of aloe vera gel application on epidermal wound healing in the domestic rabbit. Int J Res Med Sci. 2017; 5(1): 101-5. DOI: 10.18203/2320-6012.ijrms20164531

DOI: https://doi.org/10.24198/pjd.vol33no1.21276