The efficacy taro leaf extract on wound healing contaminated with Staphylococcus aureus

Endang Sjamsudin, Annisya Muharty, Lucky Riawan, Bambang Pontjo Priosoeryanto

Abstract


Introduction: Taro (Colocasia esculenta (L.) Schott) leaves have been reported to possess wound healing, anti-inflammatory, antibacterial and antioxidant activities. One of the factors that affect wound healing is infection in the wound, wherein the wound is infected by  bacteria, especially Staphylococcus aureus that is mostly found in the oral cavity, the wound healing process will be hampered and become longer healed. The objective of this research was to analyse the efficacy of Colocasia esculenta (L.) Schott extract on the healing process of wounds contaminated with Staphylococcus aureus. Methods: This experimental study on Sprague dawley rats was carried out at the Veterinary Medical Teaching Hospital of the Faculty of Veterinary Medicine of IPB University. Circular excision wounds were applied on 24 Sprague dawley rats with a diameter of 2 cm on the dorsum, and Staphylococcus aureus specimens were contaminated with a dose of 3.4x108 LAC. Rats were randomly divided into 2 groups. Group I (control group) was given a placebo, and group II (treatment group) was given a 25% concentration of Colocasia esculenta (L.) Schott extract on the wound once a day. Four rats from each group were euthanized on the 3rd, 7th, and 14th days. The number of neutrophils, macrophages, re-epithelialization and FGF-2 expression were measured by microscopic observation with visual field control, and comparative data were analysed using the Kruskal-Wallis statistical test. Results: The number of neutrophils on the treatment group on the 7th day was less than the control group (p=0.040). The number of macrophages on the 3rd day was found more in the treatment group than the control group (p=0.032), and on the 14th day, the treatment group was less than the control group (p=0.040). Epithelial cells on the 14th day of the treatment group was found more than the control group (p=.0.017). The level of FGF-2 expression of the treatment group on the 7th day was higher than the control group (p=0.044). Conclusion: Application of Colocasia esculenta (L.) Schott leaf extract is efficacious for healing wounds contaminated with Staphylococcus aureus in the proliferative phase.


Keywords


taro; Colocasia esculenta (L.) Schott; wound healing; Staphylococcus aureus; re-epithelialization; FGF-2 expression

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References


Patil BR, Ageely HM. Antihepatotoxic Activity Of Colocasia esculenta Leaf Juice. Int J Adv Biotechnol Res. 2011; 2(2): 296–304.

Agyare C, Boakye YD. Antimicrobial and Anti-Inflammatory Properties of Anchomanes difformis (Bl.) Engl. and Colocasia esculenta (L.) Schott. Biochem Pharmacol Open Access. 2015; 5:1. DOI: 10.4172/2167-0501.1000201

Pereira PR, Silva JT, Verícimo MA, Paschoalin VMF, Teixeira GAPB. Crude extract from taro (Colocasia esculenta) as a natural source of bioactive proteins able to stimulate haematopoietic cells in two murine models. J Funct Foods. 2015; 18(Part A): 333–43. DOI: 10.1016/j.jff.2015.07.014

Reinke JM, Sorg H. Wound repair and regeneration. Eur Surg Res. 2012; 49(1): 35-43. DOI: 10.1159/000339613.

Hani Sinno, Prakash S. "Complements and the Wound Healing Cascade: An Updated Review" Plastic Surgery International. 2013; Volume 2013: 7. DOI: 10.1155/2013/146764

Wilgus TA, Roy S, McDaniel JC. Neutrophils and Wound Repair: Positive Actions and Negative Reactions. Adv Wound Care (New Rochelle). 2013; 2(7): 379-88. DOI: 10.1089/wound.2012.0383.

Gonzalez ACDO, Andrade ZDA, Costa TF, Medrado ARAP. Wound healing - A literature review. An Bras Dermatol. 2016; 91(5): 614–20. DOI: 10.1590/abd1806-4841.20164741

Demidova-Rice, Tatiana N. PhD; Hamblin, Michael R. PhD; Herman, Ira M. PhD Acute and Impaired Wound Healing, Advances in Skin & Wound Care. 2012; 25(8): 349-70. DOI: 10.1097/01.ASW.0000418541.31366.a3.

Aspriyanto D, Nirwana I, Budi HS. FGF-2 expression and the amount of fibroblast in the incised wounds of Rattus norvegicus rats induced with Mauli banana (Musa acuminata) stem extract. Res Rep Dent J (Maj Ked Gigi) 2017; 50(3): 121–126. DOI: 10.20473/j.djmkg.v50.i3.p121-126

Gonzalez AC, Costa TF, Andrade ZA, Medrado AR. Wound healing - A literature review. An Bras Dermatol. 2016; 91(5): 614-20. DOI: 10.1590/abd1806-4841.20164741.

Bessa LJ, Fazii P, Di Giulio M, Cellini L. , Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection. Int Wound J, 2015. 12: 47-52. DOI: 10.1111/iwj.12049

Waasdorp M, Krom BP, Bikker FJ, van Zuijlen PPM, Niessen FB, Gibbs S. The Bigger Picture: Why Oral Mucosa Heals Better Than Skin. Biomolecules. 2021; 11(8): 1165. DOI: 10.3390/biom1108116

Amir Harriz Azmi, Siti Noor Adnalizawati Adnan & Normaliza Ab Malik .The Prevalence of Staphylococcus aureus in the Oral Cavity of Healthy Adults in Malaysia. Sains Malaysiana. 2020; 49(3): 583-91. DOI: 10.17576/jsm-2020-4903-13

Laksmitarani N, Priyanto W, Sjamsudin E. Modern Wound Dressings as Wound Care After Necrotomy Debridement at Right Submadible extended to Submentale region due to Necrotizing Fasciitis: A Case Report. J Case Rep Dent Med. 2021; 3(2): 29-33. DOI: 10.20956/jcrdm.v3i2.150

Smith R, Russo J, Fiegel J, Brogden N. Antibiotic Delivery Strategies to Treat Skin Infections When Innate Antimicrobial Defense Fails. Antibiotics. 2020; 9(2):56. DOI: 10.3390/antibiotics9020056

Tzaneva V, Mladenova I, Todorova G, Petkov D. Antibiotic treatment and resistance in chronic wounds of vascular origin. Clujul Med. 2016; 89(3): 365-70. DOI: 10.15386/cjmed-647

Gibson-Corley KN, Olivier AK, Meyerholz DK. Principles for Valid Histopathologic Scoring in Research. Veterinary Pathology. 2013; 50(6): 1007-1015. DOI: 10.1177/0300985813485099

Fedchenko N, Reifenrath J. Different approaches for interpretation and reporting of immunohistochemistry analysis results in the bone tissue - a review. Diagn Pathol. 2014; 9: 221. DOI: 10.1186/s13000-014-0221-9

Prajapati R, Kalariya M, Umbarkar R, Parmar S, Sheth N. Colocasia esculenta: A potent indigenous plant. Int J Nutr Pharmacol Neurol Dis 2011; 1(2): 90-6. DOI: 10.4103/2231-0738.84188

Sudhakar P, Thenmozhi V, Srivignesh S, Dhanalakshmi M. Colocasia esculenta (L.) Schott: Pharmacognostic and pharmacological review. J Pharmacogn Phytochem 2020; 9(4): 1382-6. DOI: 10.22271/phyto.2020.v9.i4s.11937

Chmielewski PP, Strzelec B. Elevated leukocyte count as a harbinger of systemic inflammation, disease progression, and poor prognosis: a review. Folia Morphol (Warsz). 2018; 77(2): 171-8. DOI: 10.5603/FM.a2017.0101

Ebaid H. Neutrophil depletion in the early inflammatory phase delayed cutaneous wound healing in older rats: improvements due to the use of un-denatured camel whey protein. Diagn Pathol. 2014; 9: 46. DOI: 10.1186/1746-1596-9-46

Wang J, Zhou X, Zhang Z, Xu L, Yin X, Yang L, et al. Effect of interaction of vitamin C on macrophage immune response to infection with Mycobacterium bovis. Cell Mol Biol (Noisy-le-grand). 2012; 58 Suppl: 1688–94.

Brancato SK, Albina JE. Wound macrophages as key regulators of repair: Origin, phenotype, and function. Am J Pathol. 2011; 178(1): 19–25. DOI: 10.1016/j.ajpath.2010.08.003

Koh TJ, DiPietro LA. Inflammation and wound healing: the role of the macrophage. Expert Rev Mol Med. 2011; 13: e23. DOI: 10.1017/S1462399411001943

Rousselle P, Braye F, Dayan G. Re-epithelialization of adult skin wounds: Cellular mechanisms and therapeutic strategies. Adv Drug Deliv Rev. 2019; 146: 344-65. DOI: 10.1016/j.addr.2018.06.019

Brunicardi FC. Schwartz’s Principles of Surgery, 10th Ed. McGraw Hill Medical; 2015. p. 242–64

Fonseca R. Oral & Maxillofacial Trauma. 4th Ed. Elsevier Saunders Company; 2013. p. 13–31

Nunes QM, Li Y, Sun C, Kinnunen TK, Fernig DG. Fibroblast growth factors as tissue repair and regeneration therapeutics. PeerJ. 2016; 4: e1535. DOI: 10.7717/peerj.1535

Li X, Wang C, Xiao J, McKeehan WL, Wang F. Fibroblast growth factors, old kids on the new block. Semin Cell Dev Biol. 2016; 53: 155-67. DOI: 10.1016/j.semcdb.2015.12.014

Tiara M, Januar CF, Eha D, Hanoem EH .Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schott) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture Users, Indonesian Journal of Dental Medicine. 2018; 1(1): 35-9. DOI: 10.20473/ijdm.v1i1.2018.35-39

Rima PS, Endah W, Isidora KS. Modulation of FGF2 after topical application of Stichopus hermanni gel on traumatic ulcer in Wistar rats. Dent. J. (Maj. Ked. Gigi). 2014; 47(3): 126-9. DOI: 10.20473/j.djmkg.v47.i3.p126-129




DOI: https://doi.org/10.24198/pjd.vol33no3.21325

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