Formulation of mouth rinse from the essential oils of lime (Citrus aurantifolia) and its inhibitory efficacy on the growth of Streptococcus mutans – in vitro

Sri Mulyanti; Dewi Sodja Laela; Euis Julaeha; Anne Agustina Suwargiani; Dudi Aripin

doi:10.24198/pjd.vol32no1.25486

Formulation of mouth rinse from the essential oils of lime (Citrus aurantifolia) and its inhibitory efficacy on the growth of Streptococcus mutans – in vitro

Sri Mulyanti, Dewi Sodja Laela, Euis Julaeha, Anne Agustina Suwargiani, Dudi Aripin

Abstract

Introduction: Essentials oil of lime (Citrus aurantifolia) peel has high activity in inhibiting the growth of Streptococcus mutans bacteria. The objective of this research was to determine the formulation of mouth rinse from the essential oils of lime (C. aurantifolia) with the highest inhibitory and on the growth of S. mutans. Methods: This research was an experimental in-vitro study. The sample used was the lime (C. aurantifolia) peel waste peel using hydrodistillation technique; testing of the essential oil quality was performed using 100°C distilled water for 3 hours; organoleptic test and physical mouth rinse formula test was also conducted. Mouth rinse formulations were made from the essential oils in the concentrations of 1, 2, 4, 8, 10, and 20%. Mouth rinse activity was tested with optical density. Minimum inhibitory concentration was determined with microdilution. Results: Essential oil mouth rinse formulation showed antibacterial activity at the concentrations of 2, 4, 8, 10, and 20% (formulations I-IV); the recommended mouth rinse concentration was 4% (formulation III) due to its highest antibacterial. Formulation IV showed the highest minimum inhibitory concentration value of 5.2 mg/mL (0.52%) and the safe minimum bactericidal concentration of 0.4 mg/mL (1.04). Conclusion: Mouth rinse formulation from essential oils of lime (C. aurantifolia) peel begins to show inhibition of the growth of S. mutans bacteria at the concentration of 2% essential oils (formulation II), with inhibition against the growth of S. mutans is 7.08. The formula shows a minimum inhibitory concentration (MIC) value and a minimum bactericidal concentration (MBC) of 5.2 mg/mL (0.52%) and 10.4 mg/mL (1.04), respectively.

Keywords

Lime peel, Streptococcus mutans, essential oils, Citrus aurantifolia, mouth rinse.

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References

Patil RU, Dastoor PP, Unde MP. Comparative evaluation of antimicrobial effectiveness of probiotic milk and fluoride mouthrinse on salivary Streptococcus mutans counts and plaque scores in children – An in vivo experimental study. J Indian Soc Pedod Prev Dent 2019;37(4):378-82. DOI: 10.4103/JISPPD.JISPPD_45_19

FDI World Dental Federation. The challenge of oral disease – a call for global action. The oral health atlas. 2nd ed. Geneva: FDI World Dental Federation; 2015. p. 89.

Suwargiani AA, Pribadi IMS, Hidayat W, Wardani R, Jasrin TA, Zubaedah C. Caries experience, periodontal health, and oral treatment needs of pregnant women. J Ked Gi Univ Padjadjaran. 2018;30(1):20-5. DOI: 10.24198/jkg.v30i1.16282

Raynuary N, Suwargiani AA, Suryanti N. PUFA index of pregnant women who came to the Puter Community Health Centre, Bandung, Indonesia. J Ked Gi Univ Padjadjaran. 2017;29(2):99–105. DOI: 10.24198/jkg.v29i2.18572

Gati D, Vieira AR. Elderly at greater risk for root caries: A look at the multifactorial risks with emphasis on genetics susceptibility. Int J Dent. 2011;2011:1-6. DOI: 10.1155/2011/647168

Rapp L, Maret D, Diemer F, Lacoste Ferré MH. Dental caries in geriatric dentistry: an update for clinicians. Int J Oral Dent Health. 2019; 5(1): 1–6. DOI: 10.23937/2469-5734/1510080

National Institute of Health Research and Development (NIHRD). Indonesia basic health research (RISKESDAS) 2018. Jakarta: Ministry of Health Republic of Indonesia; 2018. p. 182-3.

Salama MT, Alsughier ZA. Effect of green tea extract mouthwash on salivary streptococcus mutans counts in a group of preschool children: An in vivo study. Int J Clin Pediatr Dent. 2019;12(2):133–8. DOI: 10.5005/jp-journals-10005-1610

Budiani YA, Satari MH, Jasrin TA. Inhibitory potential of methanolic extract of pineapple, wuluh starfruit, and basil towards Streptococcus mutans ATCC 25175. J Ked Gi Univ Padjadjaran. 2017;29(2):124-30. DOI: 10.24198/jkg.v29i2.18575

Apriyanti EA, Satari MH, Laksono B. The antibacterial potential differences between the methanolic extract of ant-plant (Myrmecodia pendens Merr. & Perry) tubers and NaOCl towards Streptococcus mutans (ATCC 25175). J Ked Gi Univ Padjadjaran. 2016;28(2):106–12. DOI: 10.24198/jkg.v28i2.18704

Unita L, Singarimbun E. Antibacterial effect of the rosella flower extract towards the Streptococcus sp. colonies. J Ked Gi Univ Padjadjaran. 2018; 30(1): 64-9. DOI: 10.24198/jkg.v30i1.18199

Aripin D. Julaeha E. Efektivitas minyak atsiri dari beberapa kulit jeruk sebagai antibakteri untuk penyakit mulut dan gigi [research report]. Bandung: Universitas Padjadjaran; 2015.

Raut JS, Karuppayil SM. A status review on the medicinal properties of essential oils. Industrial Crops Products. 2014; 62: 250-64. DOI: 10.1016/j.indcrop.2014.05.055

Chouhan S, Sharma K, Guleria S. Antimicrobial activity of some essential oils—present status and future perspectives. Medicines (Basel). 2017;4(3):1-21. DOI: 10.3390/medicines4030058

Preedy VR. Essential Oils in food preservation, flavor and safety. 1st ed. Cambridge: Academic Press-Elsevier; 2016.

Dugo G, Mondello L. Citrus Oils: Composition, advanced analytical techniques, contaminants, and biological activity. Medicinal and aromatic plants - industrial profiles. Boca Raton: CRC Press; 2011.

Wahyudi T, Mulyawan AS, Kasipah C, Prayudie U, Julaeha E. Preparation of citrus aurantifolia oil microcapsules for application in textile finishing. Arena Tekst. 2017;32(1):1–8.

Chattopadhyay S, Raychaudhuri U, Chakraborty R. Artificial sweeteners - a review. J Food Sci Technol. 2014;51(4):611-21. DOI: 10.1007/s13197-011-0571-1

Barman I, Umesh CPG. Effects of Habitual arecanut and tobacco chewing on resting salivary flow rate and pH. Int J Oral Health Med Res. 2015;2(1):13-8.

Ministry of Health of the Republic of Indonesia. Farmakope Indonesia. 4th ed. Jakarta: Ministry of Health of the Republic of Indonesia; 1995.

Elwan HAM, Dawood DH, El-Shafei SMAE, El-Rahman AAEA, Abdel-Latif SA, Mohany M, et al. The potential role of citrus limon powder as a natural feed supplement to boost the productive performance, antioxidant status, and blood biochemistry of growing rabbits. Animals (Basel). 2019;9(7):426. DOI: 10.3390/ani9070426

Oliveira SA, Zambrana JR, Iorio FB, Pereira CA, Jorge AO. The antimicrobial effect of citrus limonum and citrus au-rantium essential oils on multi-species biofilm. Braz Oral Res. 2014;28:22-7. DOI: 10.1590/s1806-83242013005000024

DOI: https://doi.org/10.24198/pjd.vol32no1.25486