Profil fitokimia eco enzyme berbahan dasar kulit nanas (Ananas comosus) dan daya antibakteri terhadap Lactobacillus acidophilus ATCC 4356: Studi eksperimental

Morita Sari, Cavita Nur Imani, Astrid Pramudya Wardani

Abstract


(1) Pendahuluan: Prevalensi karies gigi, suatu penyakit mulut dan gigi, sangat tinggi di Indonesia. Kerusakan pada jaringan keras gigi, yang dipicu oleh aktivitas mikroba rongga mulut seperti Lactobacillus acidophilus, merupakan penyebab kondisi ini. Meskipun telah dikembangkan beberapa strategi pencegahan karies, sebagian besar masih bergantung pada bahan kimia, yang dapat menimbulkan risiko kesehatan jangka panjang karena kemungkinan efek sampingnya. Kulit nanas (Ananas comosus) memiliki sejumlah senyawa bioaktif yang dapat yang bertindak sebagai antibakteri alami. Tujuan penelitian ini adalah untuk mengetahui apakah kandungan kulit nanas dapat menekan atau membunuh strain Lactobacillus acidophilus ATCC 4356. (2) Metode: Penelitian ini mencakup penggunaan eksperimen laboratorium in vitro. Kelompok kontrol positif meliputi klorheksidin, sedangkan kelompok kontrol negatif berisi air suling; kelompok perlakuan meliputi eco enzyme kulit nanas pada dosis 100%, 50%, 25%, 12,5%, 6,25%, 3,12%, dan 0,78%. Zona Inhibisi (ZOI), Konsentrasi Bakterisida Minimum (MBC), dan Konsentrasi Inhibisi Minimum (MIC) ditentukan dengan melakukan uji fitokimia dan antibakteri. Analisis data dilakukan menggunakan uji Kruskal-Wallis. (3) Hasil: Enzim yang diisolasi dari kulit nanas memiliki sifat antibakteri karena adanya flavonoid, saponin, tanin, fenolik, alkaloid, steroid, antrakuinon, glukosa, dan terpenoid. Pada dosis 100%, uji ZOI menunjukkan diameter zona inhibisi yang kuat sebesar 15,51 mm. Pada konsentrasi 3,12%, nilai MIC tercapai, dan pada 6,25%, MBC ditentukan. (4) Simpulan: Eco enzyme kulit nanas (Ananas comosus) memiliki aktivitas antibakteri dalam menghambat dan membunuh Lactobacillus acidophilus ATCC 4356, sehingga berpotensi dikembangkan sebagai agen antibakteri alami untuk pencegahan karies.


Keywords


Eco_enzym; Kulit_nanas; Antibakteri; Lactobacillus_acidophilus; Fitokimia

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References


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DOI: https://doi.org/10.24198/pjdrs.v10i1.69125

DOI (PDF): https://doi.org/10.24198/pjdrs.v10i1.69125.g27447

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