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Uji efektivitas ekstrak bunga telang (Clitoria ternatea L.) sebagai alternatif disclosing agent: Studi eksperimental
Abstract
ABSTRAK
Pendahuluan: Plak gigi merupakan faktor utama terjadinya karies dan penyakit periodontal sehingga diperlukan disclosing agent untuk membantu identifikasi plak. Eritrosin sebagai disclosing agent memiliki keterbatasan, antara lain potensi toksisitas dan reaksi alergi, sehingga diperlukan alternatif berbahan alami. Bunga telang (Clitoria ternatea L.) mengandung antosianin yang berpotensi sebagai pewarna alami. Penelitian ini bertujuan menganalisis potensi ekstrak bunga telang sebagai alternatif disclosing agent dalam identifikasi plak gigi. Metode: Penelitian ini merupakan penelitian eksperimental laboratoris secara in vitro dengan rancangan post-test only control group design. Sampel penelitian terdiri atas 20 gigi premolar rahang atas hasil ekstraksi dengan menggunakan ekstrak bunga telang dengan konsentrasi 25%, 50%, 75%, dan 100% sebagai kelompok perlakuan dan eritrosin sebagai kelompok kontrol. Pengujian identifikasi plak gigi dilakukan dengan menghitung skor indeks plak metode Turesky-Gillmore-Glickman Modifikasi Quigley-Hein dengan skala maksimal 5,00 pada model plak gigi yang telah diaplikasikan ekstrak bunga telang dan eritrosin. Analisis statistik menggunakan Uji Kruskal-Wallis yang dilanjutkan dengan Uji Post-hoc Mann- Whitney. Hasil: Kadar antosianin yang didapatkan dari penelitian ini dengan menggunakan metode pH differential sebesar 933,47 mg/L. Hasil rerata skor indeks plak pada masing-masing konsentrasi ekstrak dan eritrosin secara berturut-turut sebesar 2,42; 4,08; 4,83; 5,00; dan 5,00 (p<0,05). Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan yang bermakna antara kelompok perlakuan ekstrak bunga telang konsentrasi 75% dan 100% dengan kelompok kontrol eritrosin (p>0,05). Simpulan: Ekstrak bunga telang (Clitoria ternatea L.) dengan konsentrasi 75% dan 100% berpotensi digunakan sebagai alternatif disclosing agent dari bahan alami.
KATA KUNCI: Clitoria ternatea L., disclosing agent, indeks plak Turesky-Gillmore- Glickman Modifikasi Quigley-Hein, plak gigi.
Evaluation of the effectiveness of butterfly pea (Clitoria ternatea L.) flower extract as an alternative disclosing agent: Study experimental
ABSTRACT
Introduction: Dental plaque is a major etiological factor in dental caries and periodontal disease; therefore, a disclosing agent is required to facilitate plaque identification. Erythrosine has several limitations, including potential toxicity and allergic reactions, highlighting the need for a natural alternative. Butterfly pea flower (Clitoria ternatea L.) contains anthocyanins that have the potential to serve as a natural colorant. This study aimed to analyze the potential of butterfly pea flower extract as an alternative disclosing agent for dental plaque identification. Methods: This laboratory-based experimental study was conducted in vitro using a post-test only control group design. The study samples consisted of 20 extracted maxillary premolar teeth. Butterfly pea flower extract at concentrations of 25%, 50%, 75%, and 100% was used as the treatment group treatment concentrations, while erythrosine served as the control group. Dental plaque identification was assessed by calculating plaque index scores using the Turesky–Gilmore–Glickman Modification of the Quigley–Hein Plaque Index with a maximum score of 5.00 on dental plaque models treated with butterfly pea flower extract and erythrosine. Statistical analysis was performed using the Kruskal–Wallis test followed by the Mann–Whitney post hoc test. Results: The anthocyanin content of the butterfly pea flower extract, determined using the pH differential method, was 933.47 mg/L. The mean plaque index scores for the 25%, 50%, 75%, and 100% butterfly pea flower extract groups and the erythrosine control group were 2.42, 4.08, 4.83, 5.00, and 5.00, respectively (p < 0.05). There were no statistically significant differences between the 75% and 100% butterfly pea flower extract groups and the erythrosine control group (p > 0.05). Conclusion: Butterfly pea flower (Clitoria ternatea L.) extract at concentrations of 75% and 100% has the potential to be used as a natural alternative disclosing agent for dental plaque identification.
KEY WORDS: Clitoria ternatea L, disclosing agent, dental plaque, Turesky-Gillmore-Glickman Quigley-Hein Modification, plaque index.
Keywords
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DAFTAR PUSTAKA
Kementerian Kesehatan RI, Badan Penelitian dan Pengembangan Kesehatan. Laporan Nasional Riskesdas 2018. Jakarta: Kementerian Kesehatan Republik Indonesia; 2018. p. 199-203.
Newman MG, Takei HH, Klokkevold PR, Carranza FA. Newman and Carranza's clinical periodontology. 13th ed. Philadelphia: Elsevier; 2019. p. 119-124.
Putri MH. Mikrobiologi keperawatan gigi. Pekalongan: Penerbit NEM; 2021. p. 97-98.
Febriyanti ID, Liana ID, Indriyani R, Christiono S. The effect of roselle (Hibiscus sabdariffa L.) petals extract as alternative disclosing solution for dental plaque identification. Dentino J Ked Gigi. 2018;3(2):108-15. DOI: http://dx.doi.org/10.20527/dentino.v3i2.5384.g4543
Adnan AP, Tripalu SZ, Gunawan E, Jarigau AS, Pratiwi R. Test of anthocyanin level and evaluation of rambutan leaf extract (Nephelium lappaceum L.) as an alternative to disclosing material of dental plaque detection solution. J Dentomaxillofac Sci. 2019;4(1):3-6. DOI: https://doi.org/10.15562/jdmfs.v4i1.540
Fione VR, Adam JDZ. Ekstrak ubi jalar ungu (Ipomoea batatas) sebagai solusi pewarna alamiah plak gigi. J Ilm Perawat Manado. 2021;8(2):130-41. DOI: https://doi.org/10.47718/jpd.v8i02.1197
Lee EY, Kang MK. Efficacy of self-applied plaque disclosing solution in oral care. J Korean Soc Dent Hyg. 2018;18(5):853-62.
Jung IH, Yeon KH, Song HR, Hwang YS. Cytotoxicity of dental disclosing solution on gingival epithelial cells in vitro. Clin Exp Dent Res. 2020;6(6):669-76. DOI: https://doi.org/10.1002/cre2.321.
Amaliya A, Firdaus RT, Rusminah N. Natural pigment of red-fleshed pitaya (Hylocereus polyrhizus) as dental plaque disclosing agent: a preliminary study. Istanbul J Pharm. 2020;50(3):256-61. https://doi.org/10.26650/IstanbulJPharm.2019.0066.
Marpaung AM. Tinjauan manfaat bunga telang (Clitoria ternatea L.) bagi kesehatan manusia. J Funct Food Nutraceutical. 2020;1(2):63-85. https://doi.org/10.33555/jffn.v1i2.30
Handito D, Basuki E, Saloko S, Dwikasari LG, Triani E. Analisis komposisi bunga telang (Clitoria ternatea) sebagai antioksidan alami pada produk pangan. Prosiding SAINTEK. 2022;64-70.
Angriani L. Potensi ekstrak bunga telang (Clitoria ternatea) sebagai pewarna alami lokal pada berbagai industri pangan. Canrea J Food Technol Nutr Culinary. 2019;2(1):32–37. DOI: https://doi.org/10.20956/canrea.v2i1.120
Herlina H, Jannah S, Mulyani E, Sembiring M. Analisa antosianin pada minuman olahan bunga telang (Clitoria ternatea L.) dengan metode pH diferensial. Parapemikir J Ilm Farm. 2023;12(2):217-26.
Prananta HI, Purwanto P, Misrohmasari EAA, Probosari N, Dwiatmoko S. Perbedaan indeks plak setelah pengolesan ekstrak daging buah naga merah (Hylocereus costaricensis) dan larutan pengungkap. Stomatognatic J Ked Gigi. 2019;16(1):21-4.
Halib N, Rahman NZA, Hanafiah RM, Roslan N, Jauhar NA. A simplified system for simulation of Streptococcus mutans biofilm on healthy extracted human tooth as dental plaque model. J Appl Pharm Sci. 2019;9(2):112-5. DOI: https://doi.org/10.7324/japs.2019.90215
Unawahi S, Widyasanti A, Rahimah S. Ekstraksi antosianin bunga telang (Clitoria ternatea L.) dengan metode ultrasonik menggunakan pelarut aquades dan asam asetat. J Keteknikan Pertanian Trop Biosist. 2022;10(1):1-9. DOI: https://doi.org/10.21776/ub.jkptb.2022.010.01.01
Husna A, Lubis YM, Erika C. Ekstraksi pewarna alami dari bunga telang (Clitoria ternatea L.) dengan variasi jenis pelarut dan lama ekstraksi. Jurnal Ilmiah Mahasiswa Pertanian. 2022;7(2):410-418.
Rifqi M. Ekstraksi antosianin pada bunga telang (Clitoria ternatea L.): sebuah ulasan. Pasundan Food Technol J. 2021;8(2):45-50. https://doi.org/10.23969/pftj.v8i2.4049.
Fauzi RA, Widyasanti A, Perwitasari SDN, Nurhasanah S. Optimasi proses pengeringan terhadap aktivitas antioksidan bunga telang (Clitoria ternatea) menggunakan metode respon permukaan. J Teknol Pertanian. 2022;23(1):9-22. DOI: https://doi.org/10.21776/ub.jtp.2022.023.01.2
Pramitasari R, Lim JP. Karakterisasi sifat fisikokimia ekstrak dan bubuk hasil pengeringan beku antosianin kelopak bunga telang (Clitoria ternatea L.). Agro Bali Agric J. 2022;5(2):304-12. DOI: https://doi.org/10.37637/ab.v5i2.960
Gloria Y, Delfina D, Bachtiar Y. Effectivity test antibacterial senggani leaf (Melastoma candidum) on bacteria Streptococcus mutans. J Biosains. 2019;5(1):31-7. https://doi.org/10.24114/jbio.v5i1.12333
Nayak DG, Uppoor A, Mahesh CP. Textbook of periodontology and oral implantology. 2nd ed. New Delhi: Elsevier; 2014. p. 355.
Eaton K, Ower P. Practical periodontics. Edinburgh: Elsevier; 2015. p. 98-100.
Reddy S. Essentials of clinical periodontology and periodontics. 5th ed. New Delhi: Jaypee; 2018. p. 87-92.
Netravati, Gomez S, Pathrose B, Raj MN, Joseph PM, Kuruvila B. Comparative evaluation of anthocyanin pigment yield and its attributes from butterfly pea (Clitoria ternatea L.) flowers as prospective food colorant using different extraction methods. Future Foods. 2022;6:100199. https://doi.org/10.1016/j.fufo.2022.100199.
Purwaniati P, Arif AR, Yuliantini A. Analisis kadar antosianin total pada sediaan bunga telang (Clitoria ternatea) dengan metode pH diferensial menggunakan spektrofotometri visible. J Farmagazine. 2020;7(1):18-23.
Yunianto AE, Komala R, Febriani W. Antosianin bunga telang (Clitoria ternatea L.) sebagai anti diabetes: sebuah tinjauan. Media Ilmiah Kesehatan Indonesia. 2025;3(3):135-144.
Surianti S, Husain H, Sulfikar S. Uji stabilitas pigmen merah antosianin dari daun jati muda (Tectona grandis Linn f.) terhadap pH sebagai pewarna alami. Chemica. 2019;20(1):94-101.
Almajid GAA, Rusli R, Priastomo M. Pengaruh pelarut, suhu, dan pH terhadap pigmen antosianin dari ekstrak kulit buah naga merah (Hylocereus polyrhizus). Proc Mulawarman Pharm Conf. 2021;14:179-85.
Enaru B, Drețcanu G, Pop TD, Stănilă A, Diaconeasa Z. Anthocyanins: factors affecting their stability and degradation. Antioxidants (Basel). 2021;10(12):1967.
Ayun Q, Khomsiyah, Ajeng A. Pengaruh pH larutan terhadap kestabilan warna senyawa antosianin yang terdapat pada ekstrak kulit buah naga (Hylocereus costaricensis). J Crystal. 2022;4(1):1-6.
Zayed SM, Aboulwafa MM, Hashem AM, Saleh SE. Biofilm formation by Streptococcus mutans and its inhibition by green tea extracts. AMB Express. 2021;11(1):69.
Matsumoto-Nakano M. Role of Streptococcus mutans surface proteins for biofilm formation. Jpn Dent Sci Rev. 2018;54(1):22-9.
Ouellette RJ, Rawn JD. Principles of organic chemistry. Amsterdam: Elsevier; 2015. p. 2-3.
Chen S, Wu L, Li Y, Deng W. Interaction of plum (Prunus salicina Lindl. cv. Furong) anthocyanins with Tremella polysaccharides and characteristics of their complexes. 2022;2061:1-8.
Mangiri BS, Yani S, Anitasari S. Sari buah naga super merah (Hylocereus costaricensis) sebagai pewarna alami plak gigi. J Mater Kedokt Gigi. 2018;7(1):28-34.
Sinurat F,Fadilah FM, Rosalia K, Putri L, Ariyanti D. Pengujian food colouring solutioin sebagai bahan potensial dosimeter. Scientica. 2024;2(11):480-485.
DOI: https://doi.org/10.24198/pjdrs.v10i2.69559
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