UJI AKTIVITAS ANTIBAKTERI CHITOSAN TERHADAP BAKTERI Xanthomonas campestris PV. glycines SECARA IN VITRO

Abstrak

Various kinds of soybean product technology keep expanding and have been successfully decrease pest and soybean disease. Nevertheless, after more detailed examination apparently there is a pathogen bacteria in soybean that can damaged soybean productivity, so, it can be a serious problem because they can decrease a total product of legume protein in soybean. Bacteria that usually infected in soybean is Xanthomonas campestris pv. glycines bacteria that cause lesi disease (nodule). Chitin and chitosan have multifunction like : anti-tumour, strengthen immunization, strengthen the defence toward pathogen in rat, anti-fungus, and antimicrobe. This research was done to know a kind of chitosan and also the effective concentration as the antibacterial agent toward Xanthomonas campestris pv. glycines bacteria. Chitosan that had been examined consist of 8 kinds of chitosan (FCTS (13), 2HX-40/LMCTS-500 (12), LMCTS-200 (1), LMCTS 50, LMCTS-10⁽⁸⁾, LMCTS-100, CTSTI and CTSTI substrate. Antibacterial activity of selected chitosan tested in concentration 0%, 0,001%, 0,01%, 0,025% and 0,05%. The method that use is Turbidimetri Method. Two main steps in this research are the selection step of kind of chitosan and the decision of concentrated selected chitosan. The result show that the best chitosan is a kind of CTSTI in concentration 0,05%.