Article Review: Testing for Detection of Low Pig (Porcine) DNA in Cosmetic Products and Health Supplements
Abstract
The increase in the Muslim population worldwide has led to rapid growth in the halal product industry. Currently, halal food holds the largest proportion in the global market for halal products, followed by cosmetics and health supplements as one of the main sectors. In the raw materials used for capsule shells and the manufacture of cosmetics, many ingredients come from non-halal sources, such as gelatin, fatty acids, glycerin, and collagen, which are derived from pigs. However, the presence of pork ingredients in cosmetic products and health supplements is prohibited in certain religions, such as Islam. Therefore, the detection of lard in cosmetic and pharmaceutical products plays a crucial role for the benefit of Muslim consumers. Many methods have been developed to detect pork content in highly processed products, one of which is the DNA-based method. This literature review was conducted to explain DNA isolation methods and detection techniques that can be used for pig DNA analysis. A literature search was performed through online databases, and 14 research journals were found that met the inclusion criteria. Based on the collected research, DNA-based methods, including the Polymerase Chain Reaction (PCR) and its modifications, have demonstrated the ability to detect porcine DNA in products like cosmetics and health supplements. The selection of DNA detection and isolation methods depends on the type of sample under examination, thus enhancing the accuracy and success of porcine DNA detection.
Keywords: DNA analysis, Pig DNA, Pig Content, Cosmetics, PCR, Health Supplements.
References
Hanim Yusuf A, Abdul Shukor S, Salwa Ahmad Bustamam U. Halal Certification vs Business Growth of Food Industry in Malaysia. JOEBM. 2016;4(3):247–51.
Maslul S, Utami IR. Halal Food Products Labeling According to Islamic Business Ethics and Consumers Protection Law. Al-Iktisab [Internet]. 2018 Nov 1 [cited 2023 Jun 4];2(2). Available from: https://ejournal.unida.gontor.ac.id/index.p hp/aliktisab/article/view/3900
Hermanto S, Sumarlin LO, Fatimah W. Differentiation of Bovine and Porcine Gelatin Based on Spectroscopic and Electrophoretic Analysis. JFPS. 2013;1(3).
Mukherjee S. Global Halal: Meat, Money, and Religion. Religions. 2014 Jan 29;5(1):22–75.
Marianela CR, Daniel AA. Gelatin and non-gelatin soft gel capsules: A review. 2021;
Hassan N, Ahmad T, Zain NM, Awang SR. Identification of bovine, porcine and fish gelatin signatures using chemometrics fuzzy graph method. Sci Rep. 2021 May 7;11(1):9793.
Kim YS, Yu HK, Lee BZ, Hong KW. Effect of DNA extraction methods on the detection of porcine ingredients in halal cosmetics using real-time PCR. Appl Biol Chem. 2018 Oct;61(5):549–55.
Waskitho D, Lukitaningsih E, Sudjadi, Rohman A. Analysis of Lard in Lipstick Formulation Using FTIR Spectroscopy and Multivariate Calibration: A Comparison of Three Extraction Methods. J Oleo Sci. 2016;65(10):815–24.
Lee JH, Kim MR, Jo CH, Jung YK, Kwon K, Kang TS. Specific PCR assays to determine bovine, porcine, fish and plant origin of gelatin capsules of dietary supplements. Food Chemistry. 2016 Nov;211:253–9.
Nikzad J, Shahhosseini S, Tabarzad M, Nafissi-Varcheh N, Torshabi M. Simultaneous detection of bovine and porcine DNA in pharmaceutical gelatin capsules by duplex PCR assay for Halal authentication. DARU J Pharm Sci. 2017 Dec;25(1):3.
Yang Y, Li L, Wang H, Liu M, Wu Y. Development and verification of a quantitative real‐time PCR method to identify and quantify gelatin derived from animal hide. Journal of Food Science. 2020 Sep;85(9):2762–72.
Mohamad NA, Mustafa S, El Sheikha AF, Khairil Mokhtar NF, Ismail A, Ali ME. Modification of gelatin-DNA interaction for optimised DNA extraction from gelatin and gelatin capsule: Optimised DNA extraction from gelatin. J Sci Food Agric. 2016 May;96(7):2344– 51.
Nooratiny I, Sahilah AM, Alfie ARA, Farouk MYMohd. DNA extraction from ghee and beef species identification using polymerase chain reaction (PCR) assay. IFRJ. 2013;20(5):2959–61.
Hidayati H, Hasbullah H, Hasri S. Methods for Detection of Foods, Cosmetics, and Drugs Through A Mitochondrial DNA Analysis (An Overview of the Molecular and the Qur’anic Aspects). In: Proceedings of the Proceedings of the 19th Annual International Conference on Islamic Studies, AICIS 2019, 1-4 October 2019, Jakarta, Indonesia [Internet]. Jakarta, Indonesia: EAI; 2020 [cited 2023 Apr 29]. Available from: http://eudl.eu/doi/10.4108/eai.1-10- 2019.2291746
Shabani H, Mehdizadeh M, Mousavi SM, Dezfouli EA, Solgi T, Khodaverdi M, et al. Halal authenticity of gelatin using species-specific PCR. Food Chemistry. 2015 Oct;184:203–6.
Zabidi AR, Fauzi FN, Abd Razak FN, Rosli D, Jamil MZM, Wan Ibrahim WK, et al. Screening porcine DNA in collagen cream cosmetic products. Food Res. 2020 Feb 10;4(S1):151–6.
S Abd-Gani S, Mustafa S, N Mohd Desa M, F Khairil Mokhtar N, K Hanapi U, Zakaria Z, et al. Detection of Porcine Adulteration in Cosmetic Cream Formulation via TaqMan Probe RealTime Polymerase Chain Reaction. IJET. 2019 Dec 24;7(4.14):112.
Khayyira AS, Estepane VM, Malik A. RAPID PCR–BASED DETECTION OPTIMIZATION OF PORCINE DNA IN GELATIN CAPSULE SHELL. Int J App Pharm. 2018 Nov 22;10(6):217.
Mohamad NA, Mustafa S, Khairil Mokhtar NF, El Sheikha AF. Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules: Can a molecular beacon approach be used to authenticate the origin of gelatin? J Sci Food Agric. 2018 Sep;98(12):4570–7.
Sultana S, Hossain MAM, Naquiah NNA, Ali MdE. Novel multiplex PCR-RFLP assay discriminates bovine, porcine and fish gelatin substitution in Asian pharmaceuticals capsule shells. Food Additives & Contaminants: Part A. 2018 Sep 2;35(9):1662–73.
Sultana S, Hossain MAM, Azlan A, Johan MR, Chowdhury ZZ, Ali MdE. TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements. Food Chemistry. 2020 Sep;325:126756.
Tasrip NA, Mohd Desa MN, Khairil Mokhtar NF, Sajali N, Mohd Hashim A, Ali MdE, et al. Rapid porcine detection in gelatin-based highly processed products using loop mediated isothermal amplification. J Food Sci Technol. 2021 Dec;58(12):4504–13.
Malik A, Sutantyo ML, Hapsari I, Sinurat AV, Purwati EM, Jufri M, et al. Simultaneous identification and verification of gelatin type in capsule shells by electrophoresis and polymerase chain reaction. Journal of Pharmaceutical Investigation. 2016 Aug;46(5):475–85.
Yang H, Shu Z. The extraction of collagen protein from pigskin. 2014;
Terry CF, Harris N, Parkes HC. Detection of genetically modified crops and their derivatives: critical steps in sample preparation and extraction. J AOAC Int. 2002;85(3):768–74.
Hubbard WD, Sheppard AJ, Newkirk DR, Prosser AR, Osgood T. Comparison of various methods for the extraction of total lipids, fatty acids, cholesterol, and other sterols from food products. J Amer Oil Chem Soc. 1977 Feb;54(2):81–3.
Hu Q, Liu Y, Yi S, Huang D. A comparison of four methods for PCR inhibitor removal. Forensic Science International: Genetics. 2015 May;16:94– 7.
Irine I, Nuraini H, Sumantri C. Species Authentication of Dog, Cat, and Tiger Using Cytochrome β Gene. Med Pet. 2013 Dec;36(3):171–8.
Lovatt A. Applications of quantitative PCR in the biosafety and genetic stability assessment of biotechnology products. Reviews in Molecular Biotechnology. 2002 Jan;82(3):279–300.
Mishra S, Whetstine JR. Different Facets of Copy Number Changes: Permanent, Transient, and Adaptive. Molecular and Cellular Biology. 2016 Apr 1;36(7):1050–63. 403 R. I. P. Syafitri et al / Indo J Pharm 5 (2023) 385-405
Walker JA, Hughes DA, Hedges DJ, Anders BA, Laborde ME, Shewale J, et al. Quantitative PCR for DNA identification based on genome-specific interspersed repetitive elements. Genomics. 2004 Mar;83(3):518–27.
Furda A, Santos JH, Meyer JN, Van Houten B. Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells. In: Keohavong P, Grant SG, editors. Molecular Toxicology Protocols [Internet]. Totowa, NJ: Humana Press; 2014 [cited 2023 Jun 4]. p. 419–37. (Methods in Molecular Biology; vol. 1105). Available from: http://link.springer.com/10.1007/978-1- 62703-739-6_31
Henegariu O, Heerema NA, Dlouhy SR, Vance GH, Vogt PH. Multiplex PCR: Critical Parameters and Step-by-Step Protocol. BioTechniques. 1997 Sep;23(3):504–11.
Markoulatos P, Siafakas N, Moncany M. Multiplex polymerase chain reaction: A practical approach. J Clin Lab Anal. 2002;16(1):47–51.
Hashim Ali K, Mohsin Ansari M, Ali Shah F, Ud Din F, Abdul Basit M, Kim JK, et al. Enhanced dissolution of valsartan-vanillin binary co-amorphous system loaded in mesoporous silica particles. Journal of Microencapsulation. 2019 Jan 2;36(1):10–20.
Yowani SC, Jennifer T, Ayu NCY, Putu S. Desain Pelacar DNA In Silico sebagai Pendeteksi Resistensi Fluroroquinolone pada Isolat Multi Drug Resistant Tuberculosis. Cakra Kimia (Indonesian E-Journal of Applied Chemistry). 2018;7(2).
Rahmaryani IGAAS, Ariani NK, Dewi DSW, Ani NKS, Sundari AA, Hartati KWY, et al. DNA Probe Design for Detection Mutation at Codon 315 In katG Gene of Mycobacterium Tuberculosis to Real-Time Polymerase Chain Reaction. Journal of Health Sciences and Medicin. 2017 Sep;1(2). 38. Navarro E, Serrano-Heras G, Castaño MJ, Solera J. Real-time PCR detection chemistry. Clinica Chimica Acta. 2015 Jan;439:231–50.
Lasken RS, Egholm M. Whole genome amplification: abundant supplies of DNA from precious samples or clinical specimens. Trends in Biotechnology. 2003 Dec;21(12):531–5.
Wang X, Liu Y, Liu H, Pan W, Ren J, Zheng X, et al. Recent advances and application of whole genome amplification in molecular diagnosis and medicine. MedComm [Internet]. 2022 Mar [cited 2023 Jun 4];3(1). Available from: https://onlinelibrary.wiley.com/doi/10.100 2/mco2.116
Jäger R. New Perspectives for Whole Genome Amplification in Forensic STR Analysis. IJMS. 2022 Jun 25;23(13):7090.
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