Potensi larutan teh hijau celup sebagai alternatif Hank’s Balanced Salt Solution mempertahankan viabilitas sel ligamen periodontal gigi avulsi: studi in vitro
Abstract
ABSTRAK
Pendahuluan: Avulsi gigi terjadi ketika gigi terlepas sepenuhnya dari soket alveolar akibat trauma. Perawatan awal yang dilakukan pada gigi avulsi/replantasi, yaitu menanamkan kembali gigi ke dalam soket dengan segera. Prognosis kesuksesan replantasi gigi sangat bergantung pada viabilitas sel ligamen periodontal sehingga memerlukan media penyimpanan yang sesuai dan manajemen waktu yang tepat. Penelitian bertujuan menganalisis potensi larutan teh hijau celup sebagai alternatif penggunaan Hank’s Balanced Salt Solution (HBSS) pada viabilitas sel ligamen periodontal pada gigi. Metode: Penelitian dilakukan secara eksperimental laboratoris in vitro menggunakan sampel sel ligamen periodontal pada 32 gigi insisivus maksila tikus Wistar. Sampel dibagi menjadi 8 kelompok, yaitu kelompok yang direndam dalam larutan teh hijau celup dan kelompok kontrol positif pada HBSS selama 1, 3, 6, dan 24 jam. Metode analisis penghitungan persentase viabilitas sel ligamen periodontal dengan pewarnaan eksklusi trypan blue dibawah mikroskop dengan perbesaran 100x. Analisis statistik menggunakan one way ANOVA untuk membandingkan persentase antar waktu pada teh hijau celup dan independent t-test untuk membandingkan teh hijau celup dan HBSS pada setiap waktu. Hasil: Hasil one way ANOVA p>0,05 yang menandakan bahwa tidak terdapat perbedaan signifikan antar perendaman dalam larutan teh hijau celup. Hasil independent t-test pada jam ke 1 dan 3 p>0,05 yang menandakan tidak terdapat perbedaan signifikan dan pada jam ke 6 dan 24 p<0,05 menandakan adanya perbedaan signifikan antara perendaman dalam larutan teh hijau celup dan larutan kontrol positif HBSS. Simpulan: Larutan teh hijau celup dapat menjadi alternatif HBSS dalam mempertahankan viabilitas sel ligamen periodontal gigi avulsi.
Potential of green tea bag solution as an alternative to Hank’s Balanced Salt Solution in maintaining periodontal ligament cell viability in Wistar rat avulsed teeth: in vitro study
Introduction: Tooth avulsion occurs when the tooth is completely separated from the alveolar socket due to trauma. The initial treatment for an avulsed tooth is replantation, namely immediately implanting the tooth back into the socket. The prognosis for successful tooth replantation is highly dependent on the viability of periodontal ligament cells. Use of appropriate storage media and proper time management are critical to preserving periodontal ligament cells and the likelihood of successful replantation. This study aims to analyze potential of a green tea bag solution as an alternative to using Hank’s Balanced Salt Solution (HBSS) on periodontal ligament cell viability in avulsed teeth. Methods: The research was carried out experimentally in vitro using periodontal ligament cell samples from 32 maxillary incisors of Wistar rats. The samples were divided into 8 groups, namely the group soaked in green tea bag solution and the positive control group in HBSS for 1, 3, 6, and 24 hours. The analytical method is to calculate the percentage of periodontal ligament cell viability using trypan blue exclusion staining under a microscope with 100x magnification. Statistical analysis used one way ANOVA to compare percentages between times of bagged green tea and independent t-test to compare bagged green tea and HBSS at each time. Results: The results of one way ANOVA were p>0.05 which indicated that there was no significant difference between immersion in green tea bag solution. The results of the independent t-test at the 1st and 3rd hours were p>0.05 which indicated there was no significant difference and at the 6th and 24th hours p<0.05 which indicated there was a significant difference between immersion in the green tea bag solution and HBSS as the positive control solution. Conclusion: Green tea bag solution can be an alternative to HBSS in maintaining periodontal ligament cell viability in avulsed teeth.
Keywords
Full Text:
PDFReferences
Petti S, Glendor U, Andersson L. World traumatic dental injury prevalence and incidence, a meta-analysis-One billion living people have had traumatic dental injuries. Dental Traumatology. 2018;34(2):71-86. https://doi.org/10.1111/edt.12389
Martin AG, Shivashakarappa PG, Adimoulame S, Sundaramurthy N, G E. Prevalence, Etiology, and Risk Factors of Traumatic Dental Injuries in Children with Special Needs of Puducherry. Int J Clin Pediatr Dent. 2022;15(1):104-108. https://doi.org/10.5005/jp-journals-10005-2350
Alotaibi S, Haftel A, Wagner N. Avulsed Tooth. StatPearls Publishing. National Library of Medicine. 2023. https://www.ncbi.nlm.nih.gov/books/NBK539876/
Fouad AF, Abbott P, Tsilingaridis G, Cohenca N, Lauridsen E, Bourguignon C, et al. International Association of Dental Traumatology guidelines for the management of traumatic: 2. Avulsion of permanent teeth. Dental Traumatology. 2020;36(4):331–342. https://doi.org/10.1111/edt.12573
Ali A, Al Qooz F, Mustafa, O. Tooth Avulsion : Etiology and Management. Bahrain Medical Bulletin. 2020;42(3);206-8. https://bahrainmedicalbulletin.com/SEPT_2020/SEPT2020_TOOTH.pdf
Majdiyana M, Bahar A, Darwita RR. Pengetahuan guru terkait manajemen kedaruratan gigi avulsi pada siswa sekolah dasar. Padj J Dent Res Stud. 2022;6(3):203. https://doi.org/10.24198/pjdrs.v6i3.40325
Andistiara R, Sjamsudin E, Yuza A. Tingkat pengetahuan dan pengalaman dokter gigi dalam penanganan darurat fraktur sentoalveolar. J Ked Gi Univ Padj. 2022;34(3):233-242. https://doi.org/10.24198/jkg.v34i3.39360
Demir P, Guler C, Kizilci E, Keskin G. Survival of avulsedpermanent incisors in children following delayed replantation. Niger J Clin Pract. 2020;23(63):1-7. https://doi.org/10.4103/njcp.njcp_496_19
Widyastuti A, Wardani L, Ratih DN, Widayanto PBS, Surya A, Hadriyanto W. Replantation of anterior avulsed teeth: a case report. KnE Medicine. 2020;200–207. https://doi.org/10.18502/kme.v2i1.10852
Abdelfattah P, Mostafa M, Mohamed E. Evaluation of the effect of green tea extract on periodontal ligament fibroblast viability versus Hank’s Balanced Salt Solution. Al-Azhar Dent J Girl. 2022;9(4):699-705. https://doi.org/10.21608/adjg.2022.45819.1303
Ahmed S, Kouser S, Singh G, Sharma G, Magar S, Dowad R. Comparative Evaluation of Aloe Vera, Green Tea, Histidinetryptophan Ketoglutarate Solution, and Propolis Storage Media on Viability of Periodontal Ligament Cell. Annals of R.S.C.B. 2021;11450–11458.
Inda VS, Emmanuel BJ, Singh B, Yeptho B, Shekhawat D. Storage Medium in Avulsion – Review. J Advanced Clinical & Research Insights. 2021;8(1):10-2. http://dx.doi.org/10.15713/ins.jcri.321
Rajakeerthi R, Nivedhitha MS. Natural product as the storage medium for an avulsed tooth – A systematic review. Cumhuriyet Dent J. 2019;22(2):249-256. http://dx.doi.org/10.7126/cumudj.525182
Alavi SH, Rezvani G, Esfahani MN, Lahrood FN. Periodontal ligament fibroblast cell viability following treatment with different concentrations of green tea, aloe vera and a mixture of their extracts. Front Dent. 2023;19(40):1-8. https://doi.org/10.18502/fid.v19i40.11901
Musmade DR, Mopagar V, Mopagar VP, Joshi SR, Padmai A, Pendyala G, et al. Comparative evaluation of the viability of periodontal ligaments cells in green tea and turmeric as storage media – An ex vivo study. J Cellular Biotec. 2023;9(1):57–65. http://dx.doi.org/10.3233/JCB-230089
Sheth PP, Hegde AM, Lolayekar N, Shetty V.Evaluation of Periodontal Ligament Cell Viability in Honey as a Storage Media at Different Time Intervals: An In Vitro Study. World J Dent. 2020. https://doi.org/10.5005/jp-journals-10015-1756
Sharma M, Sharma S, Reddy Y, Mittal R, Agarwal V, Singh C, Singh A. Evaluation of Periodontal Ligament Cell Viability in Three Different Storage Media: An in Vitro Study. J Dent Tehran. 2015;12(7):524-531. PMID: 26877742; PMCID: PMC4749418.
Tran S, Puhar A, Ngo-Camus M, Ramarao N. Trypan blue dye enters viable cells incubated with the pore-forming toxin hlyll of bacillus cereus. PLoS One. 2011;6(9). https://doi.org/10.1371/journal.pone.0022876
Rather S, Niveda, Karbhari S. Storage media for avulsed tooth-a review. Saudi Journal of Biomedical Research. 2020;5(11):331-334. http://dx.doi.org/10.36348/sjbr.2020.v05i11.008
Segeritz C. Vallier L. Cell culture. Chapter 9 - Cell Culture: Growing Cells as Model Systems In Vitro. Basic science methods for clinical researchers. 2017. p. 151-172. https://doi.org/10.1016/B978-0-12-803077-6.00009-6
Shen J, Ly K, Hoang Y. Cell Culture. In Basic science methods for clinical researchers. Elsevier. 2011;53–69.
Fawwaz M, Muflihunna A, Pratama M, Rahmawati R, Razak R, Baits M. Total Phenolic and Flavonoid Compound of Crude and Purified Extract of Green Tea Leaves (Camellia sinensis) from Makassar Indonesia. J Fitofarmaka Indo. 2022;9(3):19-24. https://doi.org/10.33096/jffi.v9i3.916
Musial C, Kuban-Jankowska A, Gorska-Ponikowska M. Beneficial properties of green tea catechins. Int J Mol Scie. 2020;21(5):1744. https://doi.org/10.3390%2Fijms21051744
Lorenzo J, Munekata P. Phenolic compounds of green tea : Health benefits and technological application in food. Asian Pasific Journal of Tropical Biomedicine. 2016;6(8):709-719. https://doi.org/10.1016/j.apjtb.2016.06.010
Sonoda J, Ikeda R, Baba Y, Narumi K, Kawachi A, Tomishige E, et al. Green tea catechin, epigallocatechin-3-gallate, attenuates the cell viability of human non-small-cell lung cancer A549 cells via reducing Bcl-xL expression. Exp Ther Med. 2014;8(1):59–63. https://doi.org/10.3892%2Fetm.2014.1719
Ghasempour M, Gharekhani S, Moghadamnia A, Abedian Z, Amir M, Feizi F. In vitro viability of human periodontal ligament cells in green tea extract. J Conserv Dent. 2015;18(1):47. https://doi.org/10.4103%2F0972-0707.148894
Adeli F, Zabihi E, Abedian Z, Gharekhani S, Pouramir M, Khafri S, Ghasempour M. Comparative in vitro study of the effectiveness of Green tea extract and common storage media on periodontal ligament fibroblast viability. Eur J Dent. 2016 Jul-Sep;10(3):408-412. https://doi.org/10.4103%2F1305-7456.184158
Dlugaszek M. Mierczyk, J. Elemental composition of green tea infusions depending on the method of their brewing. Europ Food Res Technol. 2024;250:301-309. http://dx.doi.org/10.1007/s00217-023-04387-7
Chacko S, Thambi P, Kuttan R, Nishigaki I. Beneficial effects of green tea : A literature review. Chinese Medicine. 2010;5(13). DOI: 10.1186/1749-8546-5-13
DOI: https://doi.org/10.24198/jkg.v36i2.55391
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Jurnal Kedokteran Gigi Universitas Padjadjaran
INDEXING & PARTNERSHIP
Jurnal Kedokteran Gigi Universitas Padjadjaran dilisensikan di bawah Creative Commons Attribution 4.0 International License