Antibacterial activity test of black betel leaf essential oil against the growth of Streptococcus mutans: Study experimental

Krisna Wahyu Wicaksono, Alhawaris Alhawaris, Sjarif Ismail

Abstract


ABSTRACT 

Introduction: The prevalence of dental caries in Indonesia remains relatively high. The main cause is Streptococcus mutans, which can proliferate and has the potential to demineralize the tooth surface. A preventive approach is the use of natural substances with antibacterial properties. One natural material with potential antibacterial activity is the essential oil of black betel leaf (DSH). This study aimed to analyze the antibacterial activity of black betel leaf essential oil against Streptococcus mutans. Methods: This study employed a true experimental design using the microdilution method. There were three groups: the test group, consisting of black betel leaf essential oil at concentrations of 4%, 2%, 1%, 0.5%, 0.25%, 0.125%, 0.0625%, 0.03125%, 0.015625%, 0.0078125%, 0.00390625%, and 0.001953125%; and the positive control group consisting of eugenol; and the negative control group consisting of 10% DMSO. Data were analyzed qualitatively by observing color changes. Results: The minimum inhibitory concentration (MIC) of black betel leaf essential oil was 0.5%. The minimum bactericidal concentration (MBC) was 4%. Columns 1A, 1B and 1C showed no color change, indicating that DSH exhibits antibacterial activity. Conclusions: Black betel leaf essential oil at a concentration of 4% exhibits the best antibacterial activity against Streptococcus mutans.

KEYWORDS: antibacterial, essential oil, black betel leaf, Streptococcus mutans

Uji aktivitas antibakteri minyak atsiri daun sirih hitam (DSH) terhadap pertumbuhan Streptococcus mutans: Studi eksperimental

ABSTRAK 

Pendahuluan: Persentase karies gigi di Indonesia masih tergolong tinggi. Penyebab utamanya berasal dari bakteri Streptococcus mutans yang dapat berkembang biak dan berpotensi dalam merusak permukaan gigi. Upaya yang dapat digunakan untuk mencegah hal tersebut, yaitu bahan alam yang berpotensi sebagai antibakteri. Salah satu bahan alam yang berpotensi sebagai agen antibakteri, yaitu minyak atsiri daun sirih hitam (DSH). Tujuan penelitian ini adalah untuk menganalisis aktivitas antibakteri minyak atsiri DSH terhadap bakteri Streptococcus mutans. Metode: Jenis metode penelitian kualitatif berupa true experimental menggunakan uji mikrodilusi. Terdapat 3 kelompok perlakuan meliputi kelompok uji minyak atsiri DSH dengan 10 konsentrasi, yaitu 4%; 2%; 1%; 0,5%; 0,25%; 0,125%; 0,0625%; 0,03125%; 0,015625%; 0,0078125%; 0,00390625% dan 0,001953125% serta kelompok kontrol positif meliputi eugenol serta kelompok kontrol negatif meliputi DMSO 10%. Analisis data dinilai secara kualitatif dengan mengamati perubahan warna. Hasil: Nilai MIC dari minyak atsiri DSH sebesar 0,5%. Nilai MBC dari minyak atsiri DSH sebesar 4%. Kolom 1A, 1B dan 1C menunjukkan tidak ada perubahan warna yang menandakan DSH bersifat antibakteri. Simpulan: Minyak atsiri DSH konsentrasi 4% memiliki aktivitas antibakteri terbaik terhadap bakteri Streptococcus mutans.

KATA KUNCI: Antibakteri, minyak atsiri, daun sirih hitam, streptococcus mutans 


Keywords


Antibakteri, minyak atsiri, daun sirih hitam, Streptococcus mutans

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DOI: https://doi.org/10.24198/pjdrs.v10i1.69075

DOI (PDF): https://doi.org/10.24198/pjdrs.v10i1.69075.g27441

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