Evaluation of Pretreatment and Enzymatic Hydrolysis Stages toward Oil Palm Empty Fruit Bunches (OPEFB) for Xylose and Glucose Production

Maisyarah Isnaini S. Nawawi, Efri Mardawati, Hana Nur Fitriana, Putri Amanda

Abstrak


Oil palm empty fruit bunches (OPEFB) as solid biomass waste from oil palm plantations have not been widely utilized due to a lack of waste handling and processing technology. OPEFB as lignocellulosic biomass consists of cellulose (44.0–43.7%), hemicellulose (22.9–23.7%), and lignin (21.3–22.1%), which have potential as feedstock materials in xylitol production. This study aimed to evaluate the pretreatment and enzymatic hydrolysis stages by deploying Cellic® Htec2 to produce high amounts of xylose and glucose. The pretreatment stage was carried out assisted by autoclave heating at 121 °C, varying the acid and base catalyst solutions (H2SO4 and NaOH), and varying the pretreatment time (15–60 min). The results showed that the pretreatment with 4% of NaOH for 15 min was able to maintain the hemicellulose content properly (23.2%). OPEFB with the highest hemicellulose content was then hydrolyzed by deploying Cellic® Htec2, which produced 12.27 g/L of xylose and 31.73 g/L of glucose. The high concentrations of xylose and glucose in OPEFB hydrolyzate show great potential to be further fermented and converted to xylitol as a low-calorie sugar alcohol.

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Referensi


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