Two-stage Enzymatics Hydrolysis for Bioethanol and Xylitol Production from Oil Palm Empty Fruits Bunches (OPEFB) as Integrated Biorefinery Implementation Strategies

Viola Caroline, Efri Mardawati, Melbi Mahardika, Hana Nur Fitriana

Abstrak


Oil palm empty fruit bunches (OPEFB) are potential waste as lignocellulosic biomass due to their abundance. The high cellulose content in the remaining OPEFB from xylitol production has great potential to be used as a material feedstock in bioethanol production. Then it encouraged further hydrolysis to be carried out in order to break down cellulose into glucose as a material feedstock in bioethanol production and to integrate the process of valorizing OPEFB, which refers to the biorefinery concept. The aims of this study were to evaluate the implementation of two-stage enzymatic hydrolysis in the preparation of reducing sugars as material feedstock for the production of integrated bioethanol and xylitol. Pretreatment was carried out as an initial stage, deploying 4% NaOH and a temperature of 121 °C for 15 minutes in an autoclave to degrade the lignin. The two-stage enzymatic hydrolysis carried out in this study used the Cellic® Htec2, which was then followed by the Cellic® Ctec2. The results showed the cellulose content in OPEFB increased to 62% after going through the pretreatment and the first stage of hydrolysis using Cellic® Htec2. The high cellulose content was then hydrolyzed to produce glucose from the first and second stages of hydrolysis, respectively at 31.73 g/L and 35.86 g/L. The pretreatment stages deployed in this study succeeded in maintaining the hemicellulose content with minimal degradation and could be hydrolyzed to xylose by as much as 12.27 g/L. The high content of glucose and xylose indicates a great potential for implementing a two-step enzymatic hydrolysis strategy, so it is promising for use in studies on the integration of bioethanol and xylitol production processes in the future.

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Referensi


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