Identifikasi Keragaman Gen Serol Regulatory Element-Binding Protein 1 (SREBP-1) sebagai Kandidat Gen untuk Kualitas Daging Pada Sapi Pasundan
Abstract
Sapi Pasundan merupakan sapi lokal yang memiliki potensi dalam memenuhi kebutuhan daging nasional khususnya di Jawa Barat. Kualitas daging salah satunya dipengaruhi oleh komposisi asam lemaknya. Sintesis asam lemak pada daging sapi dipengaruhi oleh protein yang diproduksi oleh gen SREBP-1 . Penelitian ini bertujuan untuk mengidentifikasi gen SREBP-1 sebagai kandidat gen untuk kualitas daging pada Sapi Pasundan. Metode penelitian yang digunakan adalah eksperimental eksploratif. Penelitian ini dilakukan dengan mengamplifikasi DNA dari 25 ekor Sapi Pasundan. Amplifikasi target DNA untuk mendeteksi polimorfisme 84-bp indel pada intron 5 gen SREBP-1 , dilakukan dengan teknik PCR, dengan primer spesifik. Visualisasi hasil PCR dilakukan dengan elektroforesis gel agarose. Sekuensing DNA target hasil PCR dilakukan untuk mengkonfirmasi mutasi pada gen SREBP-1 . Hasil penelitian menunjukkan adanya dua genotip, yaitu LL (96%) dan LS (4%), dengan frekuensi alel L sebesar 0,98 dan alel S sebesar 0,02. Selain itu, ditemukan dua mutasi baru, yaitu SNP g.9969T>C dan SNP g.10265A>G. Penelitian ini menunjukkan bahwa gen SREBP-1 pada Sapi Pasundan bersifat polimorfik dan berpotensi menjadi kandidat gen untuk kualitas daging yang berkaitan dengan komposisi asam lemak.
Keywords
Full Text:
PDF (Bahasa Indonesia)References
Anwar, S., Putra, W. P. B., Khaerunnisa, I., Wulandari, A. S., Prihatin, K. W., & Sutikno. (2023). The 84-bp Indel Polymorphism of The Sterol Regulatory Element-Binding Protein 1 (SREBP1) Gene in Several Cattle Breeds in Indonesia. Jurnal Ilmu Ternak Dan Veteriner, 28(2), 102–111. https://doi.org/10.14334/jitv.v28.i2.3-3046
Behjati, S., Gundem, G., Wedge, D. C., Roberts, N. D., Tarpey, P. S., Cooke, S. L., Van Loo, P., Alexandrov, L. B., Ramakrishna, M., Davies, H., Nik-Zainal, S., Hardy, C., Latimer, C., Raine, K. M., Stebbings, L., Menzies, A., Jones, D., Shepherd, R., Butler, A. P., … Zamora, J. (2016). Mutational signatures of ionizing radiation in second malignancies. Nature Communications, 7, 1–8. https://doi.org/10.1038/ncomms12605
Behling-Kelly, E., & Wong, C. (2022). Agarose gel electrophoresis determination of bovine lipoproteins compared with a wet chemistry method. JDS Communications, 3(5), 373–376. https://doi.org/10.3168/jdsc.2022-0223
Bhuiyan, M. S. A., Yu, S. L., Jeon, J. T., Yoon, D., Cho, Y. M., Park, E. W., Kim, N. K., Kim, K. S., & Lee, J. H. (2009). DNA polymorphisms in SREBF1 and FASN genes affect fatty acid composition in Korean cattle (Hanwoo). Asian-Australasian Journal of Animal Sciences, 22(6), 765–773. https://doi.org/10.5713/ajas.2009.80573
Chandrasekaran, P., & Weiskirchen, R. (2024). The Role of SCAP/SREBP as Central Regulators of Lipid Metabolism in Hepatic Steatosis. International Journal of Molecular Sciences, 25(2). https://doi.org/10.3390/ijms25021109
Cisneros-Aguirre, M., Ping, X., & Stark, J. M. (2022). To indel or not to indel: Factors influencing mutagenesis during chromosomal break end joining. DNA Repair, 118(July), 103380. https://doi.org/10.1016/j.dnarep.2022.103380
Gamarra, D., Aldai, N., Arakawa, A., de Pancorbo, M. M., & Taniguchi, M. (2019). Association of a polymorphism in SREBP1 with fatty acid composition and lipogenic gene expression in beef cattle breeds. 1–20. http://dx.doi.org/10.21203/rs.2.15242/v1
Gurr, M. I., Harwood, J. L., & Frayn, K. N. (2016). Lipid Biochemistry: An Introduction (6th ed.).
Hilmia, N., Rahmat, D., Edianingsih, P., & Faisal, Y. (2022). KOMPOSISI ASAM LEMAK PADA DAGING SAPI RANCAH DAN PERANAKAN ONGOLE. Ziraa’Ah Majalah Ilmiah Pertanian, 47(3), 425–431.
Hoashi, S., Ashida, N., Ohsaki, H., Utsugi, T., Sasazaki, S., Taniguchi, M., Oyama, K., Mukai, F., & Mannen, H. (2007). Genotype of bovine sterol regulatory element binding protein-1 (SREBP-1) is associated with fatty acid composition in Japanese Black cattle. Mammalian Genome, 18(12), 880–886. https://doi.org/10.1007/s00335-007-9072-y
Kumar, S., Singh, U., Deb, R., Tyagi, S., Mandal, D. K., Kumar, M., Sengar, G., Sharma, S., Singh, R., & Singh, R. (2015). A SNP (g.358A>T) at intronic region of CD9 molecule of crossbred bulls may associate with spermatozoal motility. Meta Gene, 5, 140–143. https://doi.org/10.1016/j.mgene.2015.07.004
Li, C., Aldai, N., Vinsky, M., Dugan, M. E. R., & McAllister, T. A. (2012). Association analyses of single nucleotide polymorphisms in bovine stearoyl-CoA desaturase and fatty acid synthase genes with fatty acid composition in commercial cross-bred beef steers. Animal Genetics, 43(1), 93–97. https://doi.org/10.1111/j.1365-2052.2011.02217.x
Li, X. Z., Yan, C. G., Gao, Q. S., Yan, Y., Choi, S. H., & Smith, S. B. (2018). Adipogenic/lipogenic gene expression and fatty acid composition in chuck, loin, and round muscles in response to grain feeding of Yanbian Yellow cattle. Journal of Animal Science, 96(7), 2698–2709. https://doi.org/10.1093/jas/sky161
Li, Y., Wu, S., Zhao, X., Hao, S., Li, F., Wang, Y., Liu, B., Zhang, D., Wang, Y., & Zhou, H. (2023). Key events in cancer: Dysregulation of SREBPs. Frontiers in Pharmacology, 14(March), 1–19. https://doi.org/10.3389/fphar.2023.1130747
Matsuda, K. (2017). PCR-Based Detection Methods for Single-Nucleotide Polymorphism or Mutation: Real-Time PCR and Its Substantial Contribution Toward Technological Refinement. In Advances in Clinical Chemistry (1st ed., Vol. 80). Elsevier Inc. https://doi.org/10.1016/bs.acc.2016.11.002
Schoech, A. P., Jordan, D. M., Loh, P. R., Gazal, S., O’Connor, L. J., Balick, D. J., Palamara, P. F., Finucane, H. K., Sunyaev, S. R., & Price, A. L. (2019). Quantification of frequency-dependent genetic architectures in 25 UK Biobank traits reveals action of negative selection. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-08424-6
Shaul, O. (2017). How introns enhance gene expression. International Journal of Biochemistry and Cell Biology, 91, 145–155. https://doi.org/10.1016/j.biocel.2017.06.016
Snyman, M., & Xu, S. (2023). The effects of mutations on gene expression and alternative splicing. Proceedings of the Royal Society B: Biological Sciences, 290(2002). https://doi.org/10.1098/rspb.2023.0565
Stenson, P. D., Mort, M., Ball, E. V., Evans, K., Hayden, M., Heywood, S., Hussain, M., Phillips, A. D., & Cooper, D. N. (2017). The Human Gene Mutation Database: towards a comprehensive repository of inherited mutation data for medical research, genetic diagnosis and next-generation sequencing studies. Human Genetics, 136(6), 665–677. https://doi.org/10.1007/s00439-017-1779-6
Suza, W., & Lee, D. (2024). GENETICS , AGRICULTURE , AND (1st ed.). Iowa State University Digital Press.
Tawaf, R. (2017). Karakteristik Usahaternak Sapi Pasundan Di Jawa Barat. Jurnal Nukleus Peternakan, 4(1), 95–101. https://doi.org/https://doi.org/10.35508/nukleus.v4i1.818
Vázquez-Mosquera, J. M., Fernandez-Novo, A., de Mercado, E., Vázquez-Gómez, M., Gardon, J. C., Pesántez-Pacheco, J. L., Revilla-Ruiz, Á., Patrón-Collantes, R., Pérez-Solana, M. L., Villagrá, A., Martínez, D., Sebastián, F., Pérez-Garnelo, S. S., & Astiz, S. (2023). Beef Nutritional Characteristics, Fat Profile and Blood Metabolic Markers from Purebred Wagyu, Crossbred Wagyu and Crossbred European Steers Raised on a Fattening Farm in Spain. Animals, 13(5). https://doi.org/10.3390/ani13050864
Wang, Y., Liu, J., HUANG, B., Xu, Y.-M., Li, J., Huang, L.-F., Lin, J., Zhang, J., MIN, Q.-H., Yang, W.-M., & Wang, X.-Z. (2015). Mechanism of alternative splicing and its regulation. Biomedical Reports, 3(2), 152–158. https://doi.org/10.3892/br.2014.407
Wen, G., Pachner, L. I., Gessner, D. K., Eder, K., & Ringseis, R. (2016). Sterol regulatory element-binding proteins are regulators of the sodium/iodide symporter in mammary epithelial cells. Journal of Dairy Science, 99(11), 9211–9226. https://doi.org/10.3168/jds.2016-11174
Wood, J. D., Enser, M., Fisher, A. V., Nute, G. R., Sheard, P. R., Richardson, R. I., Hughes, S. I., & Whittington, F. M. (2008). Fat deposition, fatty acid composition and meat quality: A review. Meat Science, 78(4), 343–358. https://doi.org/10.1016/j.meatsci.2007.07.019
DOI: https://doi.org/10.24198/jthp.v6i1.61281
Refbacks
- There are currently no refbacks.
Copyright (c) 2025 Jurnal Teknologi Hasil Peternakan
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Jurnal Teknologi Hasil Peternakan is indexed in:
In association with:
