Identifikasi Keragaman Gen FASN (Fatty Acid Synthase) sebagai Kandidat Gen Kualitas Daging pada Sapi Pasundan Betina

Abstract

Pasundan cattle are one of Indonesia’s local cattle that
have the potential to be developed, especially in terms of meat
quality. Meat quality is strongly influenced by fatty acid
composition, which is closely related to gene regulation. This study
aims to identify the diversity of the FASN (fatty acid synthase) gene
as a candidate gene for meat quality in female Pasundan cattle. This
study used an experimental descriptive method with 25 DNA
samples of female Pasundan cattle with an age range of 1,5-2 years.
DNA amplification using PCR was perfomed with specific primers
designed with NCBI Primer Blast, flanking the target gene along
560 bp. The analysis was followed by PCR-RFLP (Restriction
Fragment Length Polymorphism) method using MscI enzyme to
detect single nucleotide polymorphism (SNP) g.17924 A>G in exon
39 and confirm the target mutation using direct sequencing
method. The results showed only one genotype, the GG genotype
(100%), with a G allele frequency of 1.00. Based on these results, it
can be concluded that the FASN gene mutation g.17924 A>G
mutation in Pasundan cattle is monomorphic. SNP g.17924 A>G in
the FASN gene has the potential to affect the fatty acid composition
of Pasundan female beef